Summary
We have isolated two mutant strains, GSAr-4 and GSAr-5, which are able to grow on lactate in the presence of D-glucosamine. The glucosamine-resistant phenotype results from the cooperative effects of mutations in three loci, GAR1, GAR2 and GAR3. Both glucosamine resistant mutant strains were doubly mutant at gar1 gar2 (GSAr-4) or gar1 gar3 (GSAr-5). The mutants were also shown to exhibit glucose repression insensitive synthesis of NADH-cytochrome c reductase and cytochrome c oxidase. Glucose-repressible synthesis of the following enzymes was seen: succinic dehydrogenase, succinic: cytochrome c reductase, maltase (PNPGase), galactokinase, α-galactokinase. The glucose-repression insensitivity segregates in association with the glucosamine resistance.
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Mishra, S.D., Michels, C.A. Glucosamine-resistant mutations in yeast affecting the glucose repression sensitivity of electron transport enzymes. Curr Genet 6, 209–217 (1982). https://doi.org/10.1007/BF00390340
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DOI: https://doi.org/10.1007/BF00390340