Summary
It has been suggested that the effect of cytokinins in retarding leaf senescence comes about through their incorporation into tRNA. To test this hypothesis, kinetin-8-14C, 6-benzylaminopurine-benzyl-7-14C and adenine-8-3H were applied to detached tobacco leaves, and the nucleic acids were thereafter extracted and chromatographed on MAK columns. Kinetin-8-14C and adenine-8-3H were readily incorporated into RNA in a similar pattern. 6-Benzylaminopurine-benzyl-7-14C was effective in delaying chlorophyll loss but was not incorporated into any nucleic-acid fraction. It is concluded that the possibility of cytokinins retarding leaf senescence by completion of tRNA is not supported.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cherry, J. H., Chroboczek, H., Carpenter, J. G., Richmond, A.: Nucleic acid metabolism in peanut cotyledons. Plant Physiol. 40, 582–587 (1965).
Fittler, F., Kline, L. K., Hall, R. H.: 64-1: biosynthesis in vitro by an enzyme extract from yeast and rat liver. Biochem. biophys. Res. Commun. 31, 571–576 (1968).
Fox, J. E.: Incorporation of a Kinin, N,6-Benzyladenin, into soluble RNA. Plant Physiol. 41, 75–82 (1966).
Hall, R. H., Csonka, L., David, H., McLennan, B.: Cytokinins in the soluble RNA of plant tissues. Science 156, 69–71 (1967).
—: Isolation of N6-(γ,γ-dimethylallyl) adenosine from soluble ribonucleic acid. J. Amer. chem. Soc. 88, 2614 (1966).
Kende, H., Tavares, J. E.: On the significance of cytokinin incorporation into RNA. Plant Physiol. 43, 1244–1249 (1968).
Kulaeva, O. N.: The effect of roots on leaf metabolism in relation to the action of kinetin on leaves. [In Russ.] Fiziol. Rast. 9, 229–239 (1962).
Letham, D. S.: Regulators of cell division in plant tissues. II. A cytokinin in plant extracts: isolation and interaction with other growth regulators. Phytochemistry 5, 269–286 (1966).
—: A cytokinin in soluble RNA from a higher plant. Life Sci. 6, 387–395 (1967).
—: The structure of zeatin, a factor inducing cell division. Proc. chem. Soc. 1964, 230–231 (1964).
Linsmaier, E. M., Skoog, F.: Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127 (1965).
Mandell, J., Hershey, A.: A fractionating column for analysis of nucleic acids. Analyt. Biochem. 1, 66–67 (1960).
Miller, C. O.: Kinetin and Kinetin-like compounds. In: Modern methods of plant analysis, vol. 6 (H. F. Linskens and M. V. Tracey, eds., in coop. with B. D. Sunwal), p. 194–202. Berlin-Göttingen Heidelberg: Springer 1963.
—: Evidence for the natural occurrence of zeatin and derivatives: compounds from maize which promote cell division. Proc. nat. Acad. Sci. (Wash.) 54, 1052–1058 (1965).
Peterkofski, A.: The incorporation of mevalonic acid into the N6-(Δ 2-isopentenyl) adenosine of Transfer Ribonucleic Acid in Lactobacillus acidophilus. Biochemistry 7, 472–482 (1968).
Richmond, A. E., Lang, A.: Effect of kinetin on protein content and survival of detached Xanthium leaves. Science 125, 650–651 (1957).
Skoog, F., Armstrong, D. J., Cherayil, J. D., Hampel, A. E., Bloch, R. M.: Cytokinin activity. Localization in transfer RNA preparations. Science 154, 1354–1356 (1966).
Vanderhoef, L. N., Key, J. L.: Inhibition by kinetin of cell elongation and RNA synthesis in excised soybean hypocotyl. Plant & Cell Physiol. 9, 343–351 (1968).
Zachau, H. G., Dütting, D., Feldman, H.: Nucleotide sequences of two serinespecific transfer ribonucleic acids. Angew. Chem., Internat. edit. 5, 422 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Richmond, A., Back, A. & Sachs, B. A study of the hypothetical role of cytokinins in completion of tRNA. Planta 90, 57–65 (1969). https://doi.org/10.1007/BF00389296
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00389296