Abstract
Prothallia of Lygodium japonicum (Thunb.) Sw. were aseptically cultured under white light in a mineral solution. Solvent fractionation of the resultant culture medium and subsequent preparative thinlayer chromatography yielded a fraction that induced antheridium formation and inhibited archegonium formation. Combined gas chromatography-selected ion monitoring analysis of this fraction confirmed the presence of gibberellin A9 methyl ester (GA9-me) as an antheridiogen and an inhibitor of archegonium formation. Exogenously applied [3H]GA9 was rapidly converted to [3H]GA9-me in the prothallial tissue. Authentic GA9-me was active to 10-10M in antheridium formation and to 10-9M in the inhibition of archegonium formation.
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Abbreviations
- GAs:
-
gibberellins
- GAn :
-
gibberellin An
- GAn :
-
me, gibberellin An methyl ester
- TLC:
-
thin-layer chromatography
- GCSIM:
-
Combined gas chromatography-selected ion monitoring
References
Bray, G.A.: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Anal. Biochem. 1, 279–285 (1960)
Döpp, W.: Eine die Antheridienbildung bei Farnen fördernde Substanz in den Prothallien von Pteridium aquilinum (L.) Kuhn. Ber. Dtsch. Bot. Ges. 63, 139–147 (1950)
Döpp, W.: Über eine hemmende und eine fördernde Substanz bei der Antheridienbildung in den Prothallien von Pteridium aquilinum. Ber. Dtsch. Bot. Ges. 72, 11–24 (1959)
Emigh, V., Farrar, D.R.: Gemmae: A role in sexual reproduction in the fern genus Vittaria. Science 198, 297–298 (1977)
Hiraga, K., Yamane, H., Takahashi, N.: Biological activity of some synthetic gibberellin glucosyl esters. Phytochemistry 13, 2371–2376 (1974)
Näf, U.: On separation and identity of fern antheridiogens. Plant Cell Physiol. 9, 27–33 (1968)
Näf, U., Nakanishi, K., Endo, M.: On the physiology and chemistry of fern antheridiogen. Bot. Rev. 41, 315–359 (1975)
Nakanishi, K., Endo, M., Näf, U., Johnson, L.F.: Structure of the antheridium-inducing factor of the fern Anemia phyllitidis. J. Am. Chem. Soc. 93, 5579–5581 (1971)
Schraudolf, H.: Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien. I. Auslösung der Antheridienbildung und Dunkelkeimung bei Schizaeaceen durch Gibberellinsäure. Biol. Zentralbl. 81, 731–740 (1962)
Schraudolf, H.: Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien. IV. Die Wirkung von unterschiedlichen Gibberellinen und Allo-Gibberensäure auf die Auslösung der Antheridienbildung bei Anemia phyllitidis L. und einigen Polypodiaceen. Plant Cell Physiol. 7, 277–289 (1966)
Takeno, K., Furuya, M.: Bioassay of antheridiogen in Lygodium japonicum. Dev. Growth Differ. 17, 9–18 (1975)
Takeno, K., Furuya, M.: Inhibitory effect of Gibberellins on archegonial differentiation in Lygodium japonicum. Physiol. Plant. 39, 135–138 (1977)
Takeno, K., Furuya, M., Yamane, H., Takahashi, N.: Evidence for naturally occurring inhibitors of archegonial differentiation in Lygodium japonicum. Physiol. Plant. 45, 305–310 (1979)
Wada, M., Furuya, M.: Photocontrol of the orientation of cell division in Adiantum. I. Effects of the dark and red periods in the appical cell of gametophytes. Dev. Growth Differ. 12, 109–118 (1970)
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Yamane, H., Takahashi, N., Takeno, K. et al. Identification of gibberellin A9 methyl ester as a natural substance regulating formation of reproductive organs in Lygodium japonicum . Planta 147, 251–256 (1979). https://doi.org/10.1007/BF00388747
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DOI: https://doi.org/10.1007/BF00388747