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Zur Regulation des aeroben Intermediärstoffwechsels in ausgehungerten Hefezellen bei Zugabe von Glucose und NH4 +-Ionen

Control of aerobic metabolism in starved yeast cells supplied with glucose and ammonia

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Summary

When glucose is added to starved yeast cells under strictly aerobic conditions ([O2]>90% air saturation) the concentrations of G-6-P, F-6-P, FDP, pyruvate, malate and α-ketoglutarate rise, whereas those of PEP and PGA fall to a low level. This indicates that PK is activated and either GAPDH or PGK becomes rate limiting. Exogenous citrate is metabolized only after feeding. The level of pyruvate, α-ketoglutarate and malate accumulated is lower if ammonia was added to the medium, which indicates the onset of amino acid synthesis. The concentrations of adenosine and uridine nucleotides are diminished if ammonia is present in addition to glucose. Actinomycin prevents the decrease in adenosine nucleotide concentrations.

The accumulation of FDP and pyruvate after glucose addition is more pronounced in the presence of ammonia. This, together with changing rates of ethanol accumulation, indicates that PDC is a limiting factor during starvation and becomes activated very slowly, especially in the presence of ammonia.

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Abbreviations

ADH:

Alkoholdehydrogenase (1.1.1.1)

Ala:

L-(+)-Alanin

AMP, ADP, ATP:

Adenosin-mono-, di-, triphosphat

Asp:

L-(+)-Asparaginsäure

Asp-NH2 :

L-(+)-Asparagin

DAP:

Dihydroxyacetonphosphat

EtOH:

Äthanol

F-6-P:

β-D-Fructose-6-Phosphat

FDP:

D-Fructose-1,6-diphosphat

GAP:

D-Glycerinaldehyd-3-phosphat

GAPDH:

Glycerinaldehydphosphat-dehydrogenase (1.2.1.12)

G-6-P:

D-Glucose-6-phosphat

Glu:

L-(+) Glutaminsäure

Glu-NH2 :

Glutamin

α-Kg:

α-Ketoglutarsäure

MDH:

Malatdehydrogenase (1.1.1.37)

NADH:

Diphospho-pyridin-nucleotid, reduziert

PDC:

Pyruvatdecarboxylase (4.1.1.1)

PEP:

Phosphoenolpyruvat

PFK:

Phosphofructokinase (2.7.1.11)

PGK:

Phosphoglyceratkinase (2.7.2.3)

3-PGS, PGA:

D-3-Phosphoglycerinsäure

1,3-PGS:

D-1,3-Diphosphoglycerinsäure

PK:

Pyruvatkinase (2.7.1.40)

Pyr:

Pyruvat

Ser:

L-Serin

Thr:

L-(-)-Threonin

TRA:

Triäthanolamin

UMP:

Uridin-5′-monophosphat

UDP:

Uridin-5′-diphosphat

UTP:

Uridin-5′-triphosphat

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Authors and Affiliations

  1. Botanisches Institut der Universität, Kirschallee 1, D-5300, Bonn, Federal Republic of Germany

    S. M. Mizani, A. Betz & C. Eilers-König

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  1. S. M. Mizani
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  2. A. Betz
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  3. C. Eilers-König
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Additional information

Unter Benutzung der Dissertation von S. M. Mizani „Änderungen der Metabolitkonzentrationen in ausgehungerten Hefezellen (Saccharomyces carlsbergensis) bei Zugabe von Glucose und NH4 +-Ionen unter streng aeroben Bedingungen”, Bonn 1972.

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Mizani, S.M., Betz, A. & Eilers-König, C. Zur Regulation des aeroben Intermediärstoffwechsels in ausgehungerten Hefezellen bei Zugabe von Glucose und NH4 +-Ionen. Planta 117, 11–27 (1974). https://doi.org/10.1007/BF00388675

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