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Qualitative and quantitative analyses of gibberellins throughout seed maturation in Pisum sativum cv. Progress No. 9

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Summary

In addition to the previously identified GA20 and GA29 in immature seeds of Pisum sativum L. cv. Progress No. 9, GA9, GA17, GA38, GA44, abscisic acid and dihydrophaseic acid have been identified. The levels of GA9, GA17, GA20 and GA29 have been determined throughout seed maturation by GC-MS. GA20 and GA29 are the major gibberellins in terms of quantity, the other gibberellins remain at very low levels throughout development of the seed.

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Abbreviations

ABA:

abscisic acid

GAn :

gibberellin An

GC:

gas chromatography

GC-MS:

combined gas chromatography-mass spectrometry

GC-SICM:

combined gas chromatography-selected ion current monitoring

Me:

methyl ester

TIC:

total ion current

TLC:

thin layer chromatography

TMS:

trimethylsilyl ether

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Authors and Affiliations

  1. School of Chemistry, University of Bristol, BS8 1TS, Bristol, UK

    Valerie M. Frydman, Paul Gaskin & Jake MacMillan

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  1. Valerie M. Frydman
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  2. Paul Gaskin
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  3. Jake MacMillan
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Frydman, V.M., Gaskin, P. & MacMillan, J. Qualitative and quantitative analyses of gibberellins throughout seed maturation in Pisum sativum cv. Progress No. 9. Planta 118, 123–132 (1974). https://doi.org/10.1007/BF00388388

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  • DOI: https://doi.org/10.1007/BF00388388

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