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Release of hydrolytic enzymes from the cell walls of intact and disrupted carrot callus tissue

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Summary

The effect of ionic strength and pH on the solubility of acid invertase (E.C. 3.2.1.26.) and α-glucosidase (E.C. 3.2.1.20.) from carrot Daucus carota L. tissue cultures is presented. The action of these two factors on disrupted tissue is contrasted with the effects of the same treatment on the solubility of these enzymes from intact tissue. The results are discussed in terms of a unified theory on the solubility behaviour of acid invertase and other hydrolytic enzymes in plant cells.

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Abbreviations

CRT II:

CRT II carrot callus (Hanson a. Edelman, 1971)

References

  • Arnold, W. N.: Heat inactivation kinetics of yeast β-fructofuranosidase. A polydisperse system. Biochim. biophys. Acta (Amst.) 178, 347–353 (1969)

    Google Scholar 

  • Chang, C. W., Bandurski, R. S.: Exocellular enzymes of corn root. Plant Physiol. 39, 60–64 (1964)

    Google Scholar 

  • Chang, P. L. Y., Trevithick, J. R.: How important is secretion of exoenzymes through apical cell walls in fungi? Arch. Microbiol. 101, 281–293 (1974)

    PubMed  Google Scholar 

  • Copping, L. D., Street, H. E.: Properties of the invertase of cultured Sycamore cells and changes in their activity during culture growth. Physiol. Plant. 26, 246–254 (1972)

    Google Scholar 

  • Edelman, J., Hall, M. A.: Enzyme formation in higher-plant tissues. Development of invertase and ascorbate oxidase activities in mature storage tissue of Helianthus tuberosus L. Biochem. J. 95, 403–410 (1965)

    PubMed  Google Scholar 

  • Eylar, E. H.: On the biological role of glycoproteins J. theoret. Biol. 10, 89–113 (1965)

    Google Scholar 

  • Gayler, K. R., Galaszion, K. T.: Physiological functions of acid and neutral invertases in growth and sugar storage in sugar cane. Physiol. Plant. 27, 25–31 (1972)

    Google Scholar 

  • Hanson, A. D., Edelman, J.: Secretion of photosynthetic products by carrot tissue cultures. Planta (Berl.) 98, 97–108 (1971)

    Google Scholar 

  • Hawker, J. S.: Insoluble invertase from grapes: an artifact of extraction. Phytochemistry 8, 337–344 (1969)

    Article  Google Scholar 

  • Hawker, J. S.: Enzymes concerned with sucrose synthesis and transformations in seeds of maize, broad bean and castor bean. Phytochemistry 10, 2313–2322 (1971)

    Article  Google Scholar 

  • Jansen, E. F., Jang, R., Bonner, J.: Binding of enzymes to Avena coleoptile cell walls Plant Physiol. 35, 567–574 (1960)

    Google Scholar 

  • Jaynes, T. A., Haskins, F. A., Gorz, H. J., Kleinhofs, A.: Solubility of β-glucosidase in homogenates of sweetclover leaves and bean hypocotyls. Plant Physiol. 49, 277–279 (1972)

    Google Scholar 

  • Keegstra, K., Albersheim, P.: Involvement of glycosidase in cell wall metabolism of suspension cultured Acer pseudoplatanus cells. Plant Physiol. 45, 675–678 (1970)

    Google Scholar 

  • Klis, F. M.: α-Glucosidase activity located at the cell surface in Convolvulus arvensis callus. Physiol. Plant. 25, 253–257 (1971)

    Google Scholar 

  • Klis, F. M., Akster, R. A.: Acid cell-wall invertases in Convolvulus callus. Phytochemistry 13, 1737–1740 (1974)

    Article  Google Scholar 

  • Little, G., Edelman, J.: Solubility of plant invertases. Phytochemistry 12, 67–71 (1973)

    Article  Google Scholar 

  • Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with Folin-phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    PubMed  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497 (1962)

    Google Scholar 

  • Nakagawa, H., Sekiguchi, K., Ogura, N., Takehana, H.: Binding of tomato pectinesterase and β-fructofuranosidase to tomato cell wall. Agric. Biol. Chem. 35, 301–307 (1971)

    Google Scholar 

  • Parr, D. R.: Ph. D. Thesis, University of London (1974)

  • Reinert, J., Backs, D.: Control of totipotency in plant cells growing in vitro. Nature (Lond.) 220, 1340–1341 (1968)

    Google Scholar 

  • Ricardo, C. P. P., apRees, T.: Invertase activity during the development of carrot roots. Phytochemistry 9, 239–247 (1970)

    Article  Google Scholar 

  • Somogyi, M.: Notes on sugar determination. J. biol. Chem. 195, 19–23 (1952)

    PubMed  Google Scholar 

  • Thorpe, T. A., Meier, D. D.: Sucrose metabolism during tobacco callus growth. Phytochemistry 12, 493–497 (1973)

    Article  Google Scholar 

  • Ueda, Y., Ishiyama, H., Fukui, M., Nishi, A.: Invertase in cultured Daucus carota cells. Phytochemistry 13, 383–387 (1974)

    Article  Google Scholar 

  • Vaughan, D., MacDonald, I. R.: Development of soluble and insoluble invertase activity in washed storage tissue slices. Physiol. 42, 456–458 (1967)

    Google Scholar 

  • Waheed, A., Shall, S.: Relationship between carbohydrate content and enzymatic activity of yeast invertase. Enzymologia 41, 291–303 (1971)

    PubMed  Google Scholar 

  • Weinberg, R., Orton, W. L.: Elution of exocellular enzymes from Saccharomyces fragilis and S. cervisiae. J. Bact. 91, 1–13 (1966)

    PubMed  Google Scholar 

  • Wray, J. L., Brice, R. E.: Location and density labelling of acid invetase in aging discs of Daucus carota storage tissue. Phytochemistry 12, 1917–1921 (1973)

    Article  Google Scholar 

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Author notes

  1. D. R. Parr

    Present address: Department of Biophysics, University College London, Gower Street, WC1E 6BT, London, UK

  2. J. Edelman

    Present address: The Lord Rank Research Centre, Lincoln Road, HP1Z 3QR, High Wycombe, Bucks, UK

Authors and Affiliations

  1. Biology Department, Queen Elizabeth College, Campden Hill, W8 7AH, London, UK

    D. R. Parr & J. Edelman

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  1. D. R. Parr
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  2. J. Edelman
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Parr, D.R., Edelman, J. Release of hydrolytic enzymes from the cell walls of intact and disrupted carrot callus tissue. Planta 127, 111–119 (1975). https://doi.org/10.1007/BF00388372

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  • DOI: https://doi.org/10.1007/BF00388372

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