Skip to main content
Log in

Transfer of algal chloroplasts into protoplasts of higher plants

  • Published:
Planta Aims and scope Submit manuscript

Summary

Polyethylene-glycol treatment of mixed suspensions of isolated protoplasts and of chloroplasts induces chloroplast uptake by the protoplasts. Chloroplasts of algal origin (Vaucheria dichotoma (L.) Ag.) were transferred with high frequency into protoplasts of Daucus carota L.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Carlson, P. S.: Towards a parasexual cycle in higher plants. In: Protoplasts et fusion de cellules somatiques végétales. Coll. Intern. C.N.R.S. No. 212, pp. 497–505 (1973a)

  • Carlson, P. S.: The use of protoplasts for genetic research. Proc. nat. Acad. Sci. (Wash.) 70, 598–602 (1973b)

    Google Scholar 

  • Davey, M. R., Cocking, E. C.: Uptake of bacteria by isolated higher plant protoplasts. Nature (Lond.) 239, 455–456 (1972)

    Google Scholar 

  • Eriksson, T., Bonnett, H. T., Glimelius, K., Wallin, A.: Technical advances in protoplast isolation, culture and fusion. In: Advances in plant science through tissue culture, Street, H. E., ed. New York-London: Acad. Press, (in press) 1975

    Google Scholar 

  • Glimelius, K., Wallin, A., Eriksson, T.: Agglutinating effects of Concanavalin A on isolated protoplasts of Daucus carota. Physiol. Plant. 30, 564–569 (1974)

    Google Scholar 

  • Kao, K. N., Michayluk, M. R.: A method for high-frequency intergeneric fusion of plant protoplasts. Planta (Berl.) 115, 355–367 (1974)

    Google Scholar 

  • Nass, M. M. K.: Uptake of isolated chloroplasts by mammalian cells. Science 165, 1128–1131 (1969)

    PubMed  Google Scholar 

  • Potrykus, I.: Transplantation of chloroplasts into protoplasts of Petunia. Z. Pflanzenphysiol. 70, 364–366 (1973)

    Google Scholar 

  • Potrykus, I., Hoffman, F.: Transplantation of nuclei into protoplasts of higher plants. Z. Pflanzenphysiol. 69, 287–289 (1973)

    Google Scholar 

  • Trench, R. K., Gooday, G. W.: Incorporation of [3H]-leucine into protein by animal tissues and by endosymbiotic chloroplasts in Elysia viridis Montagu. Comp. biochem. Physiol. 44A, 321–330 (1973)

    Article  Google Scholar 

  • Wallin, A., Eriksson, T.: Protoplast cultures from cell suspensions of Daucus carota. Physiol. Plant. 28, 33–39 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bonnett, H.T., Eriksson, T. Transfer of algal chloroplasts into protoplasts of higher plants. Planta 120, 71–79 (1974). https://doi.org/10.1007/BF00388272

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388272

Keywords

Navigation