Abstract
Pyrus malus L. var. Golden delicious embryos were incubated with (±)-[2-14C]abscisic acid (ABA) (10-5 M, 355 kBq μmol-1). After incubations of various durations, the radioactivity was measured in whole embryos, cotyledons, and embryonic axes.
With either 48-h or 16-d incubation periods, the uptake of [14C]ABA depended upon the mode of culture used. The lowest values corresponded to the absorption by the embryonic axis, the highest to the absorption by the distal parts of the two cotyledons. The cotyledons accumulated the main part of the radioactivity under all conditions. Dormant and almost completely after-ripened embryos cultivated for 4 d showed no significant differences in the radioactivity uptake for identical modes of culture. There was a linear relationship between exogenous ABA concentrations (0.5 to 3.10-5 M) and ABA uptake for embryos cultivated for 4 d with the distal parts of the cotyledons immersed in the medium.
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Abbreviations
- ABA:
-
abscisic acid. RM, RM+, C/2 M, and CM are different modes of embryo cultures: embryonic axis immersed alone (RM), together with the proximal parts of the cotyledons (RM+); distal parts of the cotyledons immersed alone (CM); embroyo flat on the medium, the root and the external surface of one cotyledon being in contact with the medium (C/2 M)
- PP:
-
proximal parts of the cotyledons
- DP:
-
distal parts of the cotyledons
References
Côme, D., Thévenot, C. (1970) La dormance des embryons de Pommier (Pirus malus L.) en relation avec l'imbibition et l'intensité respiratoire de leurs cotylédons. Bull. Acad. Soc. Lorr. Sc. 9, 42–58
Durand, M., Thévenot, C., Côme, D. (1975) Rôle des cotylédons dans la germination et la levée de dormance de l'axe embryonnaire de Pommier, après traitement par l'acide abscissique. Physiol. Vég. 13, 603–611
Kaminski, W., Rudnicki, R., Pieniazek, J. (1971) Some growth responses of apple seedlings to abscisic acid and growth stimulators. Biol. Plant. 13, 128–132
Le Page-Degivry, M.Th., Bulard, C. (1979) Acide abscissique lié et dormance embryonnaire chez Pyrus malus. Physiol. Plant. 46, 115–120
Rudnicki, R. (1969) Studies on abscisic acid in apple seeds. Planta 86, 63–68
Rudnicki, R., Kaminski, W., Pieniazek, J. (1971) The interaction of abscisic acid with growth stimulators in germination of partially after-ripened apple embryos. Biol. Plant. 13, 122–127
Thévenot, C. (1980) Corrélations entre les cotylédons et l'axe de l'embryon de Pommier. Interprétation de la dormance. Thèse Sc. Nat. Paris VI, pp. 327
Thévenot, C., Côme, D. (1971) Influence de la température et du mode d'imbibition sur la germination des embryons de Pommier (Pirus malus L.) non dormants. C. R. Acad. Sci. Paris 273, 2515–2517
Thévenot, C., Côme, D. (1973a) Mise en évidence de l'influence des cotylédons sur la levée de dormance et la germination de l'axe de l'embryon de Pommier (Pirus malus L.). Physiol. Vég. 11, 161–169
Thévenot, C., Côme, D. (1973b) Inhibition de la germination de l'axe embryonnaire par les cotylédons chez le Pommier (Pirus malus L.). C.R. Acad. Sci. Paris 277, 1873–1876
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Barthe, P., Bulard, C. Uptake of [2-14C]abscisic acid and distribution of 14C in apple embryos. Planta 152, 336–340 (1981). https://doi.org/10.1007/BF00388258
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DOI: https://doi.org/10.1007/BF00388258