Abstract
Micrococcal nuclease digestion of chromatin from ungerminated and 48 h-germinated pea embryos yields DNA fragments which are multiples of basic units of 194–195 base pairs. Extensive digestion produces a core particle of 145 base pairs. Deoxyribonuclease I gives rise to fragments which are multiples of 10 bases upon analysis on denaturing gels. These values are comparable with those found for other plant materials. These results indicate that gross changes in nucleosomal organization do not accompany the onset of germination.
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Axel, R., Melchior, W., Sollner-Webb, B., Felsenfeld, G.: Specific sites of interaction between histones and DNA in chromatin. Proc. Natl. Acad. Sci. USA 71, 4101–4105 (1974)
Burton, K.: Determination of DNA concentration with diphenylamine, In: Methods in Enzymology, vol. XII, pt B, pp. 163–166. Grossman, L., Moldave, K., eds. New York: Academic Press 1968
Cheah, K.S.E., Osborne, D.J.: Analysis of nucleosomal deoxyribonucleic acid in a higher plant. Biochem. J. 163, 141–144 (1977)
Camerini-Otero, R.D., Sollner-Webb, B., Felsenfeld, G.: The organization of histones and DNA in chromatin: evidence for an arginin-rich histone kernel. Cell 8, 333–347 (1976)
Dubochet, J., Ducommun, M., Zollinger, M., Kellenberger, E.: A new preparation method for dark-field electron microscopy of biomacromolecules. J. Ultrastruct. Res. 35, 147–167 (1971)
Frado, L.L.Y., Annunziato, A.T., Woodcock, C.L.F.: Structural repeating units in chromatin. III. A comparison of chromatin subunits from vertebrate, ciliate and angiosperm species. Biochim. Biophys. Acta 475, 514–520 (1977)
Gigot, C., Philipps, G., Nicolaieff, A. Hirth, L.: Some properties of tobacco protoplast chromatin. Nucl. Acids Res. 3, 2315–2329 (1976)
Grellet, F., Delseny, M., Guitton, Y.: Histone content of germinating pea embryo chromatin decreases as DNA replicates. Nature (London) 267, 724–726 (1977)
Kornberg, R.D.: Structure of chromatin. Annu. Rev. Biochem. 46, 931–954 (1977)
Leammli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 277, 680–685 (1970)
Lebeurier, G., Withechurch, O., Lesot, A., Hirth, L.: Physical Map of DNA from a new cauliflower mosaic virus strain. Gene 4, 213–226 (1978)
Lohr, D., Corden, J., Tatchell, K., Kovacic, R.T., Van Holde, K.E.: Comparative subunit structure of Hela, yeast, and chicken erythrocyte chromatin. Proc. Natl. Acad. Sci. USA 74, 79–83 (1977)
Lowry, O.H., Rosebrough, N.J., Farr, J.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Maniatis, T., Jeffrey, A., van de Sande, H.: Chain length determination of small double-and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14, 3787–3794 (1975)
Martin, D.Z., Todd, R.D., Lang, D., Pei, P.N., Garrard, W.T.: Heterogeneity in nucleosome spacing. J. Biol. Chem. 252, 8269–8277 (1977)
McGhee, J.D., Engel, J.D.: Subunit structure of chromatin is the same in plants and animals. Nature (London) 254, 449–450 (1975)
Morris, R.: Nucleosome structure in Aspergillus nidulans. Cell 8, 357–363 (1976)
Noll, M.: Internal structure of the chromatin subunit. Nucl. Acids Res. 1, 1573–1578 (1974)
Noll, M., Thomas, J.O., Kornberg, R.D.: Preparation of native chromatin and damage caused by shearing. Science 187, 1203–1206 (1975)
Noll, M.: Differences and similarities in chromatin structure of Neurospora crassa and higher eucaryotes. Cell 8, 349–355 (1976)
Noll, M., Kornberg, R.D.: Action of Micrococcal nuclease on chromatin and the location of histone H1. J. Mol. Biol. 109, 393–404 (1977)
Oudet, P., Gross-Bellard, M., Chambon, P.: Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell 4, 281–300 (1975)
Peacock, A.C., Dingman, C.W.: Molecular Weight estimation and separation of ribonucleic acid by electrophoresis in agarose acrylamide composite gels. Biochemistry 7, 668–674
Philipps, G., Gigot, C.: DNA associated with nucleosomes in plants. Nucl. Acids Res. 4, 3617–3626 (1977)
Spiker, S., Isenberg, I.: Cross-complexing pattern of plant histones. Biochemistry 16, 1819–1825 (1977)
Tatchell, K., van Holde, K.E.: Compact oligomers and nucleosome phasing. Proc. Natl. Acad. Sci. USA 75, 3583–3587 (1978)
Voordouw, G., Eisenberg, H.: Binding of additional histones to chromatin core particles. Nature (London) 273, 446–448 (1978)
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Grellet, F., Penon, P. & Cooke, R. Analysis of DNA associated with nucleosomes in pea chromatin. Planta 148, 346–353 (1980). https://doi.org/10.1007/BF00388122
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DOI: https://doi.org/10.1007/BF00388122