Abstract
Gibberellic acid (GA3) stimulates isocitrate lyase activity of the endosperm during germination of castor bean seeds. Isocitrate lyase from castor bean was purified and an antibody to it was prepared from rabbit serum. This antibody was used to measure the amounts of isocitrate lyase-mRNA using an in vitro translation system. No specific stimulation of isocitrate lyase-mRNA by application of GA3 was detected. The stimulation of isocitrate lyase activity by exogenous GA3 may be accounted for by the action of the growth substance in advancing the overall production of rRNA and mRNA which accelerates the rate of total protein synthesis during germination. The application of Amo 1618 retards the production of isocitrate lyase activity but also retards protein synthesis in general. This suggests that endogenous gibberellins also act non-specifically in the regulation of protein synthesis during castor bean germination.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- SDS:
-
sodium dodecyl sulphate
- GA3 :
-
gibberellic acid
- PAGE:
-
polyacrylamide gel electrophoresis
References
Beevers, H. (1969) Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis. Ann. N.Y. Acad. Sci.168, 313–324
Ben Tal, Y., Varner, J.E. (1974) An early response to gibberellic acid not requiring protein synthesis. Plant Physiol.54, 813–816
Bowden, L., Lord, J.M. (1976) Similarities in the polypeptide composition of gloxysomal and endoplasmic-reticulum membranes from castor bean endosperm. Biochem. J.154, 491–499
Bowles, D.J., Kauss, H. (1976) Characterisation, enzymatic and lectin properties of isolated membranes from Phaseolus aureus. Biochim. Biophys. Acta.443, 360–374
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem.72, 248–252
Carpenter, W.D., Beevers, H. (1959) Distribution and properties of isocitratase in plants. Plant Physiol.34, 403–409
Choinski, J.S. (1981) Gibberellins and the development of glyoxalate cycle enzyme activity in castor bean endosperm. Plant Physiol.67 (Suppl.), 149
Chrispeels, M.J., Varner, J.E. (1967) Gibberellic acid enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol.42, 398–406
Evins, W.H., Varner, J.E. (1971) Hormone-controlled synthesis of endoplasmic reticulum in barley aleurone cells. Proc. Natl. Acad. Sci. USA68, 1631–1633
Frevert, J., Kindl, H. (1978) Plant microbody proteins. Eur. J. Biochem.92, 35–43
Gerhardt, B., Beevers, H. (1970) Developmental studies on glyoxysomes in Ricinus endosperm. J. Cell Biol.44, 94–102
Gientka-Rychter, A., Cherry, J.A. (1968) De novo synthesis of isocitratase in peanut (Arachis hypogaea L.) cotyledons. Plant Physiol.43, 653–659
Gonzalez, E. (1978) Effect of gibberellin A3 on the endoplasmic reticulum and on the formation of glyoxysomes in the endosperm of germinating castor bean. Plant Physiol.62, 449–453
Hock, B., Beevers, H. (1966) Development and decline of the glyoxalate cycle enzymes in water melon seedlings (Citrullus vulgaris Schrad.) Z. Pflanzenphysiol.55, 405–414
Johnson, K.D., Kende, H. (1971) Hormonal control of lecithin synthesis in barley aleurone cells: regulation of the CDP-Choline pathway by gibberellin. Proc. Natl. Acad. Sci. USA68, 2674–2677
Khan, F.R., Saleemuddin, M., Siddiqui, M., McFadden, B.A. (1977) Purification and properties of isocitrate lyase from flax seedlings. Arch. Biochem. Biophys.183, 13–23
Koehler, D.E., Varner, J.E. (1973) Hormonal control of orthophosphate incorporation into phospholipids of barley aleurone layers. Plant Physiol.52, 208–214
Köller, W., Kindl, H. (1977) Glyoxalate cycle enzymes of the glyoxysomal membrane from cucumber cotyledons. Arch. Biochem. Biophys.181, 236–248
Lamb, J.E., Riezman, H., Becker, W.M., Leaver, C.J. (1978) Regulation of glyoxysomal enzymes during germination of cucumber. Plant Physiol.62, 754–760
Laskey, R.A., Mills, A.D. (1975) Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur. J. Biochem.56, 335–341
Laskey, R.A., Mills, A.D., Gurdon, J.B., Partington, G.A. (1977) Protein synthesis in oocytes ofXenopus laevis is not regulated by the supply of messenger RNA. Cell11, 345–351
Lockard, R.E., Lingrel, J.B. (1972) Mouse haemoglobin messenger ribonucleic acid. J. Biol. Chem.247, 4174–4179
Lodish, H.F., Jacobsen, M. (1972) Regulation of haemoglobin synthesis. J. Biol. Chem.247, 3622–3629
Marriott, K.M., Northcote, D.H. (1975) The induction of enzyme activity in the endosperm of germinating castor bean seeds. Biochem. J.152, 65–70
Marriott, K.M., Northcote, D.H. (1977) The influence of abscisic acid, adenosine 3′, 5′ cyclic phosphate and gibberellic acid on the induction of isocitrate lyase activity in the endosperm of germinating castor bean seeds. J. Exp. Bot.28, 219–224
Martin, C.R., Northcote, D.H. (1981) Qualitative and quantitative changes in mRNA of castor beans during the initial stages of germination. Planta151, 189–197
Martin, C.R., Northcote, D.H. (1982) The action of exogenous gibberelic acid on protein and mRNA in germinating castor bean seeds. Planta154, 168–173
Mollenhauer, H.H., Totten, C. (1970) Studies on seeds. Plant Physiol.46, 794–799
Mozer, T.J. (1980) Control of protein synthesis in barley aleurone layers by the plant hormones gibberellic acid and abscisic acid. Cell20, 479–485
Naylor, J.M. (1966) Dormancy studies in seed ofAvena fatua. Can. J. Bot.44, 19–32
Schröder, J. (1977) Light-induced increase og messenger RNA for phenylalanine ammonia-lyase in cell suspension cultures ofPetroselinum hortense. Arch. Biochem. Biophys.182, 488–496
Schröder, J., Kreuzaler, F., Schäfer, E., Hahlbrock, K. (1979) Concomitant induction of phenylalanine ammonia-lyase and flavone synthase mRNAs in irradiated plant cells. J. Biol. Chem.254, 57–65
Reeve, D.R., Crozier, A. (1975) Gibberellin bioassays. In: Gibberellins and plant growth. Krishnamoorthy, H.N., ed. Halsted Press
Veeger, C., der Vartanian, D.V., Zeylemaker, W.P. (1969) Succinate dehydrogenase. Methods Enzymol.13, 81–90
Vigil, E.L. (1970) Cytochemical and developmental changes in microbodies (glyoxysomes) and related organelles of castor bean endosperm. J. Cell Biol.46, 435–454
Weir, E.M., Riezman, H., Grienenberger, J.M., Becker, W.M., Leaver, C.J. (1980) Regulation of glyoxysomal enzymes during germination of cucumber. Eur. J. Biochem.112, 469–477
Wrigley, A., Lord, J.M. (1977) The effects of gibberellic acid on organelle biogenesis in the endosperm of germinating castor bean seeds. J. Exp. Bot.28, 345–353
Youle, R.J., Huang, A-H. (1976) Protein bodies from the endosperm of castor bean. Plant Physiol.58, 703–709
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Martin, C., Northcote, D.H. The action of exogenous gibberellic acid on isocitrate lyase —mRNA in germinating castor bean seeds. Planta 154, 174–183 (1982). https://doi.org/10.1007/BF00387913
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00387913