Abstract
Heterotrophically grown Euglena synthesize grains of paramylon, its reserve carbohydrate, in a vesicular complex of mitochondrial origin. A CO2 fixation activity in dark grown Euglena was demonstrated in the mitochondria via paramylon. At the beginning of the exponential phase of growth, the activity of phosphoenolpyruvate carboxykinase increases before the augmentation of paramylon.
At the end of the exponential phase, the activity of this enzyme decreases, and low residual levels persist in the transition and stationary phases of growth. The activity of phosphoenolpyruvate carboxylase evolves inversely during the heterotrophic growth of the algae in succinate- or a lactate-containing medium. A compartmentalized scheme of carbon metabolism in mitochondria is presented.
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Abbreviations
- PEP:
-
phosphoenolpyruvate
- OAA:
-
oxaloacetate
- PGA:
-
phosphoglyceric acid
References
Barras, D.R., Stone, B.A. (1968) Carbohydrate composition and metabolism in Euglena. In: Biology of Euglena, pp. 149–191 Buetow E., ed., Academic Press. New York and London
Bray, G.A. (1960) A simple effluent liquid scintillation method for counting aqueous solutions in a liquid scintillation counter. Anal. Biochem. 1, 279–285
Briand, J., Calvayrac, R. (1980) Paramylon synthesis in heterotrophic and photoheterotrophic Euglena. J. Phycol. 16, 234–239
Calvayrac, R. (1970) Relation entre les subtrats, la respiration et la structure mitochondriale chez Euglena gracilis Z. Arch. Mikrobiol. 73, 308–314
Calvayrac, R. (1972) Le cycle des mitochondries chez Euglena gracilis en culture synchrone. Thèse de Doctorat, Univ. Paris XI
Calvayrac, R., Ledoigt, G. (1976) Croissance des Euglènes en présence de DCMU: Evolution du plastidome en fonction de la tension d'oxygène. Plant Sci. Lett. 7, 249–263
Calvayrac, R., Levy, M., Vazart, J., Briand, J., Claisse, M. (1978) Activité cytochrome oxydase des mitochondries d'Euglena gracilis Z: Comparaison de l'action du ferrocytochrome c 558 et due ferrocytochrome c de mammifères. Biol. Cell, 31, 141–148
Calvayrac, R., Briand, J. (1978) Paramylon synthesis and the chondriome of Euglena gracilis Z. In: Plant mitochondria, pp. 435–443 G. Ducet and C. Lance, eds. Elsevier North Holland
Calvayrac, R., Bomsel, J.L., Laval-Martin, D. (1979) Analysis and characterization of DCMU resistant Euglena. I. Growth, metabolic and ultrastructural modifications during adaptation to different doses of DCMU. Plant Physiol. 63, 857–865
Codd, G.A., Merrett, M.J. (1971) Phosphopyruvate carboxylase activity and carbon dioxide fixation via C4 acids over the division cycle in synchronized Euglena cultures. Planta 100, 124–130
Colman, B., Cheng, K.H., Ingle, R.K. (1976) The relative activities of phosphoenol-pyruvate carboxylase and ribulose di-phosphate carboylase in blue-green algae. Plant Sci. Lett. 6, 123–127
Cook, J.R. (1965) Influence of light on acetate utilization in green Euglena. Plant Cell Physiol. 6, 301–307
Conn, E.E., Stumpf, P.K. (1976) Outlines of Biochemistry (1976) John Wiley and sons Pub. pp. 294
Cramer, M.L., Myers, J. (1952) Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol. 17, 384–402
Dittrich, P., Campbell, W.H., Black, C.C. (1973) Phosphoenol pyruvate carboxykinase in plants exhibiting Crassulacean acid metabolism. Plant Physiol. 52, 357–361
Drobnica, L., Ebringer, L. (1962) The determination of the activity of some enzymes of carbon metabolism in extracts of Euglena gracilis cells. Folia Microbiol. (Prague) 8, 56–59
Edwards, G.E., Kanai, R., Black, C.C. (1971) Phosphoenol pyruvate carboxykinase in leaves of certain plants with fix CO2 by the C4 dicarboxylic acid cycle of photosynthesis. Biochem. Biophys. Res. Comm. 45, 278–285
Jomain-Baum, M., Schramm, V.L., Hanson, R.W. (1976) Mechanism of 3-mercaptopicolinic acid inhibition of hepatic phosphoenolpyruvate carboxykinase (GTP) J. Biol. Chem. 251, 37–44
Karn, R.C., Kivic, P.A., Hudock, G.A. (1973) A procedure for the electrophoretic analysis of PEPcase. Biochim. Biophys. Acta 293, 567–569
Laval-Martin, D., Farineau, J., Diamond, J. (1977) Light versus dark carbon metabolism in cherry Tomato fruits. I occurence of photosynthesis. Study of the intermediates. Plant Physiol. 60, 872–876
Leegood, R.C., Rees, T. (1978) Dark fixation of CO2 during gluconeogenesis by the cotyledons of Cucurbita pepo L. Planta 140, 275–282
Leveldahl, B.H. (1966) Heterotrophic CO2 fixation by a bleached Euglena. Exp. Cell Res 44, 393–402
Levedahl, B.H. (1967) The effect of CO2 on succinate metabolism in a bleached Euglena. Exp. Cell Res. 48, 125–131
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275
Lynch, V.H., Calvin, M. (1953) CO2 fixation by Euglena Ann. N.Y. Acad. Sci., 56, 890
Mazelis, M., Vennesland, B. (1957) Carbon dioxide fixation into oxalacetate in higher plants. Plant Physiol. 32, 591–600
Morot-Gaudry, J.F., Farineau, J. (1978) Etude comparée des réactions de carboxylations photosynthétiques chez un Maïs normal (W 64 A) et chez un Maïs mutant opaque 2 (W 64 A o 2): mise en évidence de déviations métaboliques chez le mutant. Physiol. Vég. 16, 451–467
Ohmann, E., Plhâk, F. (1969) Reinigung und Eigenschaften von Phosphoenolpyruvat-case aus Euglena gracilis. Eur. J. Biochem. 10, 43–55
Perl, M. (1974) Phosphoenolpyruvate carboxylating enzyme in dark-grown and in green Euglena cells. J. Biochem. 76, 1095–1101
Wolpert, J.S., Ernst-Fonberg, M.L. (1975) A multi enzymes complex for CO2 fixation. Biochemistry 14, 1095–1102
Wolpert, J.S., Ernst-Fonberg, M.L. (1975) Dissociation and characterization of enzymes from a multi-enzymes complex involved in CO2 fixation. Biochemistry 14, 1103–1107
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Briand, J., Calvayrac, R., Laval-Martin, D. et al. Evolution of carboxylating enzymes involved in paramylon synthesis (phosphoenolpyruvate carboxylase and carboxykinase) in heterotrophically grown Euglena gracilis . Planta 151, 168–175 (1981). https://doi.org/10.1007/BF00387819
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DOI: https://doi.org/10.1007/BF00387819