Skip to main content
SpringerLink
Log in

Hydroxyproline: its asymmetric distribution in a cell wall glycoprotein

  • Published:
Planta Aims and scope Submit manuscript

Abstract

The main structural glycoprotein of the cell wall of Chlamydomonas reinhardii has been cleaved by thermolysin into glycopeptides which have been separated into three fractions, T1, T2 and T3. These correspond to three distinct domains within the glycoprotein, characterized by the asymmetric distribution of both sugars and amino acids, in particular hydroxyproline. T2 is very rich in hydroxyproline (43 mol %) and is highly glycosylated, while T3 is poor in hydroxyproline and contains very little carbohydrate. The results are discussed in terms of cell wall glycoproteins and their function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PAGE:

polyacrylamide gel electrophoresis

Tris:

Tris(hydroxymethyl)-methylamine

SDS:

Sodium dodecyl sulphate

PAS:

periodic acid-Schiff

References

  • Bhatti, T., Chambers, R.E., Clamp, J.R.: The gas chromatographic properties of biologically important N-acetyl-glucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides. Biochim. Biophys. Acta. 222, 339–347 (1970)

    PubMed  Google Scholar 

  • Brown, R.G., Kimmins, W.C.: Glycoproteins in int. rev. biochem. Plant Biochemistry II, Vol. 13, pp. 183–209. Northcote, D.H., ed. Baltimore 1977

  • Catt, J.W., Hills, G.J., Roberts, K.: A structural glycoprotein containing hydroxyproline, isolated from the cell wall of Chlamydomonas reinhardii. Planta 131, 165–171 (1976)

    Google Scholar 

  • Catt, J.W., Hills, G.J., Roberts, K.: Cell wall glycoproteins from Chlamydomonas reinhardii and their self assembly. Planta 138, 91–98 (1978)

    Google Scholar 

  • Delmer, D.P., Lamport, D.T.A.: The origin and significance of plant glycoproteins. In: Cell Wall Biochemistry Related to Specificity in Host-plant Pathogen Interactions, pp. 85–102, Solheim, B., Raa, J., eds. Tromso 1976

  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for the determination of sugars and related substances. Anal. Chem. 28, 350–356 (1956)

    Google Scholar 

  • Heath, M.F., Northcote, D.H.: Glycoprotein of the wall of sycamore tissue culture cells. Biochem. J. 125, 953–961 (1971)

    PubMed  Google Scholar 

  • Heinrikson, R.L.: Applications of thermolysin in protein structural analysis. Methods Enzymol. 47, 175–189 (1977)

    PubMed  Google Scholar 

  • Homer, R., Roberts, K.: Glycoprotein conformation in plant cell walls: circular dichroism reveals a polyproline II conformation (1979)

  • Lamport, D.T.A., Miller, D.H.: Hydroxyproline arabinosides in the plant kingdom. Plant Physiol. 48, 454–456 (1971)

    Google Scholar 

  • Lamport, D.T.A.: The role of hydroxyproline-rich proteins in the extracellular matrix of plants. Symp. Soc. Dev. Biol. 30, 113–130 (1974)

    PubMed  Google Scholar 

  • Lamport, D.T.A.: Structure, biosynthesis and significance of cell wall glycoproteins. Recent Adv. Phytochem. 11, 79–115 (1977)

    Google Scholar 

  • Matsubara, H.: Purification and assay of thermolysin. Methods Enzymol. 19, 642–651 (1970)

    Google Scholar 

  • Miller, D.H., Lamport, D.T.A., Miller, M.: Hydroxyproline heterooligosaccharides in Chlamydomonas. Science 176, 918–920 (1972)

    PubMed  Google Scholar 

  • Miller, D.H., Mellman, I.S., Lamport, D.T.A., Miller, M.: The chemical composition of the cell wall of Chamydomonas gymnogama and the concept of a plant cell wall protein. J. Cell. Biol. 63, 420–429 (1974)

    PubMed  Google Scholar 

  • Rapaka, R.S., Renugopalakrishnan, V., Urry, D.W., Bhatnagar, R.S.: Hydroxylation of proline in polytripeptide models of collagen: stereochemistry of polytripeptide-prolyl hydroxylase interaction. Biochemistry 17, 2892–2898 (1978)

    PubMed  Google Scholar 

  • Roberts, K.: Chrystalline glycoprotein cell walls of algae: their structure, composition and assembly. Philos. Trans. R. Soc. London, Ser., B 268, 129–146 (1974)

    Google Scholar 

  • Tautvydas, K.J.: Isolation and characterization of an extracellular hydroxyproline-rich glycoprotein and a mannose-rich polysaccharide from Eudorina californica (Shaw). Planta 140, 213–220 (1978)

    Google Scholar 

  • Wang, H.M., Chan, J., Pettigrew, D.W., Sodek, J.: Cleavage of native type III collagen in the collagenase susceptible region by thermolysin. Biochem. Biophys. Acta 533, 270–277 (1978)

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K.

    Keith Roberts

Authors
  1. Keith Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This is the seventh paper in a series entitled ‘Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardii”. The last paper in this series was Catt et al. (1978)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roberts, K. Hydroxyproline: its asymmetric distribution in a cell wall glycoprotein. Planta 146, 275–279 (1979). https://doi.org/10.1007/BF00387798

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00387798

Key words

Search

Navigation