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Transport de l'auxine-14C en provenance de jeunes gousses de Vicia faba L.

Transport of [14C] auxin from young pods of Vicia faba L.

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Summary

After the injection of [14C]indole acetic acid (IAA) into very young pods of broad-bean (Vicia faba L.) the movement of the 14C in the peduncle and stem was followed by autoradiography. In samples with only one young pod the basipetal transport was always clearly dominant. Most of the radioactivity was found in the bundles, particularly in the outer region of the bundle and also in the inner region (protoxylem parenchyma). The progression of the tracer was relatively complex. The rate of movement of the radioactive «front» could be as much as 2 cm·h-1 but most of the 14C moved towards the base at rates clearly less than that of the «front». Chromatograms with several solvent systems showed that IAA was the main or the only mobile radioactive substance. During transport, a part of IAA was converted into indole-3-aldehyde (IAld) and indole-3-acetyl-aspartic acid (IAAsp). IAAsp and possibly also IAld, which were found mainly near the donor pod, seemed immobile. This work is part of a study on the interchange of phytohormones between fruit and plant.

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Abbreviations

AIA:

acide indolyl-acétique

AIAsp:

acide indolyl-acetyl-aspartique

IAld:

indolyl-aldéhyde

Références

  • Antoszewski, R., Lis, E.: Translocation of some radioactive compounds from the strawberry receptacle to the mother plant. Bull. Acad. pol. Sci. 16, 443–446 (1968)

    Google Scholar 

  • Blumenfeld, A., Gazit, S.: Cytokinin activity in avocado seeds during fruit development. Plant Physiol. 46, 331–333 (1970)

    Google Scholar 

  • Bonnemain, J. L.: Anatomie et physiologie de l'appareil conducteur des Solanacées. Thèse Sc. nat., Paris (1968)

  • Bonnemain, J. L.: Transport de l'AIA marqué et de ses dérivés à partir de jeunes fruits. C. R. Acad. Sci. (Paris) (D) 270, 1326–1329 (1970a)

    Google Scholar 

  • Bonnemain, J. L.: Histogenèse du phloème interne et du phloème inclus des Solanacées. Rev. gén. Bot. 77, 5–51 (1970b)

    Google Scholar 

  • Bonnemain, J. L.: Transport et distribution des traceurs après application de AIA-2-14C sur les feuilles de Vicia faba. C. R. Acad. Sci. (Paris) (D) 273, 1699–1702 (1971)

    Google Scholar 

  • Bonnemain, J. L., Bourbouloux, A.: The transport and metabolism of 14C-indole-acetic-acid in intact plants. 3rd Symposium on accumulation and translocation of nutrients and regulators in plant organisms, Warszawa, in press (1973)

  • Bourbouloux, A., Bonnemain, J. L., Vasseur, J.: Caracteristiques du transport dans la tige de l'auxine-14C en provenance de jeunes feuilles de Vicia faba L. C. R. Acad. Sci. (Paris) (D) 276, 501–504 (1973)

    Google Scholar 

  • Goldsmith, M. H.: Movement of pulses of labelled auxin in corn coleoptiles. Plant Physiol. 42, 258–263 (1967)

    PubMed  Google Scholar 

  • Graebe, J. E., Dennis, D. T., Upper, C. D., West, C. A.: Biosynthesis of gibberellins. I. The biosynthesis of (-)—kaurene, (-)—kauren-19 and trans-geranylgeraniol in endosperm nucellus of Echinocystis macrocarpa Greene. J. biol. Chem. 240, 1847–1854 (1965)

    PubMed  Google Scholar 

  • Grochowska, M. J.: Translocation of indole-3-acetic acid-2-14C injected into seeds of five-week-old apple fruits. Bull. Acad. pol. Sci. 16, 577–580 (1968)

    Google Scholar 

  • Hall, S. M., Baker, D. A.: The chemical composition of Ricinus phloem exudate. Planta (Berl.) 106, 131–140 (1972)

    Google Scholar 

  • Hoad, G. V., Bowen, M. R.: Evidence for gibberellin-like substances in phloem exudate of higher plants. Planta (Berl.) 82, 22–32 (1968)

    Google Scholar 

  • Hoad, G. V., Hillman, S. K., Wareing, P. F.: Studies on the movement of indole auxins in willow (Salix viminalis L.). Planta (Berl.) 99, 73–88 (1971)

    Google Scholar 

  • Hollis, Ch. A., Tepper, H. B.: Auxin transport within intact dormant and active white ash roots. Plant Physiol. 48, 146–149 (1971)

    Google Scholar 

  • Homan, D. N.: Auxin transport in the physiology of fruit development. Plant Physiol. 39, 982–986 (1964)

    Google Scholar 

  • Huber, B., Schmidt, E., Jahnel, H.: Untersuchungen über den Assimilatstrom. I. Mitteilung aus der Sächsischen Forstlichen Versuchsanstalt Tharandt, Abteilung für Botanik. Tharandt. Forstl. Jahrb. 88, 1017–1050 (1937)

    Google Scholar 

  • Kende, H.: Preservation of chlorophyll in leaf sections of substances obtained from root exudate. Science (N.Y.) 145, 1066–1067 (1964)

    Google Scholar 

  • Kende, H.: Kinetin like factors in the root exudate of sunflowers. Proc. nat. Acad. Sci. (Wash.) 53, 1302–1307 (1965)

    Google Scholar 

  • Kluge, M., Reinhard, E., Ziegler, H.: Gibberellin-Aktivität von Siebröhrensäften. Naturwissenschaften 6, 145–146 (1964)

    Google Scholar 

  • Kondratieva-Melville, H. A.: On the influence of the developing bean in the stem bundles of Vicia faba L. Bot. Zh. 56, 1605–1609 (1971)

    Google Scholar 

  • Lenz, F.: Der Fruchtstiel als Indikator für Wuchsstofftransport aus der Frucht. Mitt. Rebe Wein Obstbau Früchteverwertg. 21, 327–330 (1971)

    Google Scholar 

  • Letham, D. S., Williams, M. W.: Regulators of cell division in plant tissues. VIII. The cytokinins of the apple fruit. Physiol. Plant. 22, 925–936 (1969)

    Google Scholar 

  • Lis, E. K., Antoszewski, R.: Translocation of IAA-14COOH and some mineral ions in the strawberry peduncle. Zesz. Nauk. Univ. M. Kopernika, Nauki Matem.-Przyrodn., Toruń, Biologia 13, 175–179 (1970)

    Google Scholar 

  • Morris, D. A., Briant, R. E., Thomson, P. G.: The transport and metabolism of 14C-labelled indoleacetic acid in intact pea seedlings. Planta (Berl.) 89, 178–197 (1969)

    Google Scholar 

  • Newman, I. A.: Auxin transport in Avena. I. Indoleacetic acid-14C distributions and speeds. Plant Physiol. 46, 263–272 (1970)

    Google Scholar 

  • Nitsch, J. P.: Perennation through seeds and other structures: fruit development. In: Plant physiology, a treatise, vol. VIA, p. 413–501, ed. Steward, F. C. New York-London: Academic Press 1971

    Google Scholar 

  • Persson, A., Rappaport, L.: Gibberellin-induced systemic fruit set in a male-sterile tomato. Science 127, 816 (1958)

    Google Scholar 

  • Phillips, D. A., Cleland, C. F.: Cytokinin activity from the phloem sap of Xanthium strumarium L. Planta (Berl.) 102, 173–178 (1972)

    Google Scholar 

  • Phillips, I. D. J., Jones, R. L.: Gibberellin-like activity in bleeding sap of root systems of Helianthus annuus detected by a new dwarf pea epicotyl assay and other methods. Planta (Berl.) 63, 269–278 (1964)

    Google Scholar 

  • Reid, D. M., Burrows, W. J.: Cytokinin and gibberellin-like activity in spring sap of trees. Experientia (Basel) 24, 189–190 (1968)

    Google Scholar 

  • Sachar, R. C., Kapoor, M.: Gibberellin in the induction of parthenocarpy in Zephyranthes. Plant. Physiol. 34, 168–170 (1959)

    Google Scholar 

  • Skene, K. G. M.: Gibberellin-like substances in root exudate of Vitis vinifera. Planta (Berl.) 74, 250–262 (1967)

    Google Scholar 

  • Smid, N., Vardjan, M.: Les cytokinines dans la sève printanière du bouleau Betula pendula Roth. Biol. Vest. 18, 27–36 (1970)

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Physiologie de la Conduction et de la Nutrition végétale, U.E.R. de Biologie, Université des Sciences et Techniques de Lille, B.P. 36, F-59650, Villeneuve d'Ascq, France

    Andrée Bourbouloux & Jean-Louis Bonnemain

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  1. Andrée Bourbouloux
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  2. Jean-Louis Bonnemain
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Bourbouloux, A., Bonnemain, JL. Transport de l'auxine-14C en provenance de jeunes gousses de Vicia faba L.. Planta 115, 161–172 (1973). https://doi.org/10.1007/BF00387781

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  • DOI: https://doi.org/10.1007/BF00387781

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