Abstract
A technique has been developed for the enzymatic isolation of leaf cells from the Crassulacean acid-metabolism plant Sedum telephium. The cells exhibited high activity in both 14CO2 incorporation (30–70 μmol CO2 mg-1 chlorophyll h-1) and O2 evolution in the presence of bicarbonate (60–110 μmol O2 mg-1 chlorophyll h-1). Half-maximum saturation of 14CO2 incorporation occurred at a bicarbonate concentration of ca. 2 mM (20 μM CO2) at pH 8.4 and 30°C. Two types of light-dependent O2 evolution are reported: O2 evolution in the absence of exogenously supplied bicarbonate (endogenous O2 evolution), and bicarbonate-stimulated O2 evolution. Oxygen evolution in the presence of approximately ambient concentrations of CO2 appeared to be a combination of the endogenous O2 evolution and O2 evolution from fixation of the exogenously supplied CO2.
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Abbreviations
- CAM:
-
Crassulacean acid metabolism
- cirlo:
-
chlorophyll
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- PEP:
-
phosphoenolpyruvate
- RuDP:
-
ribulose-1,5-diphosphate
References
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Spalding, M.H., Edwards, G.E. Photosynthesis in enzymatically isolated leaf cells from the CAM plant Sedum telephium L.. Planta 141, 59–63 (1978). https://doi.org/10.1007/BF00387745
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DOI: https://doi.org/10.1007/BF00387745