Summary
Callose was detected by fluorescence microscopy in megasporogenesis in all investigated species with mono- and bisporic embryo-sac development. Callose occurs first in the meiotic prophase in the chalazal part of the megasporocyte wall and by the first meiotic metaphase the whole cell is enveloped in a callose-containing wall. Later, there is a marked decrease of callose fluorescence, usually at the chalazal end of the megasporocyte. In Oenothera, where the micropylar megaspore is active, decrease of fluorescence takes place at the micropylar pole of the megasporocyte. Callose appears centrifugally in the cell plates forming eventually the walls dividing the megaspores. It disappears from the walls of the megaspores during degeneration and differentiation.
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References
Albertini, L.: Etude autoradiographique des synthèses de protèines au cours de la microsporogénèse chez le Rhoeo discolor. C. R. Acad. Sci. (Paris) 264D, 2773 (1967).
Currier, H. B.: Callose substance in plant cells. Amer. J. Bot. 44, 478–488 (1957).
Engelman, E. M.: Sieve element of Impatiens sultanii. I. Wound reaction. Ann. Bot. 29, 83–101 (1965).
Eschrich, W.: Kallose. Protoplasma 47, 487–530 (1956).
—, Currier, H. B.: Identification of callose by dichrome and fluorochrome reactions. Stain. Technol. 39, 303–308 (1964).
Heslop-Harrison, J.: Cell walls, cell membranes and protoplasmic connections during meiosis and pollen development. In: Pollen physiology and fertilisation (H. F. Linskens, ed.), p. 39–47. Amsterdam: North-Holland 1964.
—: Cytoplasmic continuities during spore formation in flowering plants. Endeavour 25 (95), 65–72 (1966).
—, Mackenzie, A.: Autoradiography of soluble (2-14C)-thymidine derivatives during meiosis and microsporogenesis in Lilium anthers. J. Cell. Sci. 2, 387–400 (1967).
Mangin, L.: Observation sur la membrane du grain de pollen mûr. Bull. Soc. Bot. France 36, 274–284 (1889).
Rodkiewicz, B.: Walls with callose in the megaspores and hypostase of ovules of Antirrhinum majus observed in a fluorescence microscope. Bull. Acad. Polon., Cl. V, 15, 493–495 (1967).
—: Differences in the distribution pattern of callose in cell walls during megasporogenesis in some species of flowering plants. Bull. Acad. Polon., Cl. V, 16, 663–665 (1968).
—, Górska-Brylass, A.: Ocurrence of callose in the walls of meiotically dividing cells in the ovule of Orchis. Naturwissenschaften 54, 499 (1967).
——: Callose in the walls of the developing megasporocyte and megaspores in the orchid ovule. Acta Soc. Bot. Polon. 37, 19–28 (1968).
Sauter, J. J.: Histoautographische Untersuchungen zur Ribonucleinsäure-Synthese während der Meiosis bei Paeonia tenuifolia L. Naturwissenschaften 55, 236 (1968).
Sloover, J. L. de: Etudes sur les Cycadales. I. Méiose et mégasporogénèse chez Encephalartos poggei Asch. Cellule 62, 103–116 (1961).
Taylor, J. H.: Autoradiographic studies of nucleic acids and proteins during meiosis in Lilium longiflorum. Amer. J. Bot. 46, 477–484 (1959).
Waterkeyn, L.: Etude des dépots de callose au niveau des parois sporocytaires au moyen de la microscopie de fluorescence. C. R. Acad. Sci. (Paris) 252, 4025 (1961).
Weiling, F.: Zur Feinstruktur der Plasmodesmen und Plasmakanäle bei Pollen-mutterzellen. Planta (Berl.) 64, 97–118 (1965).
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Rodkiewicz, B. Callose in cell walls during megasporogenesis in angiosperms. Planta 93, 39–47 (1970). https://doi.org/10.1007/BF00387650
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DOI: https://doi.org/10.1007/BF00387650