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Vacuolar protein bodies in tomato leaf cells and their relationship to storage of chymotrypsin inhibitor I protein

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Summary

Electron microscopy of leaves of tomato has shown that tissue containing chymotrypsin inhibitor I protein has protein in the cell vacuoles. The vacuolar protein was found either as many small bodies or as few large bodies. The data indicate that the vacuole is a temporary storage site for protein which may play an important role in growth and development of the plant. This strongly suggests that the plant-cell vacuole is something more than a site for terminal deposition of waste products. The system offers an unusual opportunity to study the biochemistry and ultrastructure of synthesis, vacuolar deposition, and recall of a well-characterized plant protein.

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Authors and Affiliations

  1. Washington State University, Washington, USA

    L. K. Shumway, J. M. Rancour & C. A. Ryan

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  1. L. K. Shumway
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  2. J. M. Rancour
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  3. C. A. Ryan
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Additional information

This work was supported in part by Washington State Medical and Biological funds, U.S. Public Health Service Grants 2K3GM17059 and GM12505, and by U.S.D.A. C.S.R.S. Grant No. 915-15-29. College of Agriculture scientific paper 3442, Projects 1791 and 1996.

Program in Genetics and Department of Botany.

Department of Agricultural Chemistry.

Department of Agricultural Chemistry. Research Career Development Awardee of the U.S. Public Health Service.

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Shumway, L.K., Rancour, J.M. & Ryan, C.A. Vacuolar protein bodies in tomato leaf cells and their relationship to storage of chymotrypsin inhibitor I protein. Planta 93, 1–14 (1970). https://doi.org/10.1007/BF00387647

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  • DOI: https://doi.org/10.1007/BF00387647

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