Summary
The development of increased activities of ribulosediphosphate carboxylase (EC 4.1.1.39) and of phosphoribulokinase (EC 2.7.1.19) in greening bean leaves was completely inhibited by D-threo chloramphenicol but unaffected by L-threo chloramphenicol. This indicates that these enzymes are synthesized by the ribosomes of the developing plastids. A different mechanism appears to be responsible for the development of activity of NADP-dependent triosephosphate dehydrogenase (EC 1.2.1.13) where the D-threo isomer gave 45% inhibition and the L-threo isomer gave 18% inhibition. Thus both specific (D-threo isomer) and unspecific (both isomers) inhibition occurred. It is suggested that the development of NADP-dependent triosephosphate dehydrogenase activity may result from the allosteric activation, in the plastids, of the NAD-dependent enzyme (Müller et al., 1969) which has been synthesized by cytoplasmic ribosomes. Neither isomer inhibited the development of five other enzymes of the photosynthetic carbon cycle namely ribosephosphate isomerase (EC 5.3.1.6), phosphoglycerate kinase (EC 2.7.2.3), triosephosphate isomerase (EC 5.3.1.1), tructosediphosphate aldolase (EC 4.1.2.13) and transketolase (EC 2.2.1.1), but there was a significant stimulation of the activity of transketolase by D-threo chloramphenicol.
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References
Berger, C., Feierabend, J.: Plastidenentwicklung und Bildung von Photosynthese-Enzymen in etiolierten Roggenkeimlingen. Physiol. Vég. 5, 109–122 (1967).
Bradbeer, J. W.: The activities of the photosynthetic carbon cycle enzymes of greening bean leaves. New Phytologist 68, 233–245 (1969).
Bradbeer, J. W.: Plastid development in primary leaves of Phaseolus vulgaris. The effect of short blue, red, far-red and white light treatments on dark-grown plants. J. exp. Bot. 22, in the press (1971).
— Clijsters, H., Gyldenholm, A. O., Edge, H. J. W.: Plastid development in primary leaves of Phaseolus vulgaris. The effects of brief flashes of light on darkgrown plants. J. exp. Bot. 21, 525–533 (1970).
Bradbeer, J. W. Gyldenholm, A. O., Wallis, M. E., Whatley, F. R.: Studies in the biochemistry of chloroplast development. In: Progress in Photosynthesis Research (Tübingen) 1, 272–279 (1969).
Chen, S., McMahon, D., Bogorad, L.: Early effects of illumination on the activity of some photosynthetic enzymes. Plant Physiol. 42, 1–5 (1967).
Ellis, R. J.: Chloroplast ribosomes: stereo-specificity of inhibition by chloramphenicol. Science 163, 477–478 (1969).
Feierabend, J.: Enzymbildung in Roggenkeimlingen während der Umstellung von heterotrophem auf autotrophes Wachstum. Planta (Berl.) 71, 326–355 (1966).
Graham, D., Hatch, M. D., Slack, C. R., Smillie, R. M.: Light-induced formation of enzymes of the C4-dicarboxylic acid pathway of photosynthesis in detached leaves. Phytochem. 9, 521–532 (1970).
Gyldenholm, A. O., Whatley, F. R.: The onset of photophosphorylation in chloroplasts isolated from developing bean leaves. New Phytologist 67, 461–468 (1968).
Keller, C. J., Huffaker, R. C.: Evidence for in vivo light-induced synthesis of ribulose-1,5-diP carboxylase and phosphoribulokinase in greening barley leaves. Plant Physiol. 42, 1277–1283 (1967).
Kirk, J. T. O.: Studies on the dependence of chlorophyll synthesis on protein synthesis in Euglena gracilis, together with a nomogram for determination of chlorophyll concentration. Planta (Berl.) 78, 200–207 (1968).
Latzko, E., Gibbs, M.: Distribution and activity of enzymes of the reductive pentose phosphate cycle in spinach leaves and in chloroplasts isolated by different methods. Z. Pfl. Physiol. 59, 184–194 (1968).
Margulies, M. M.: Effect of chloramphenicol on light dependent development of seedlings of Phaseolus vulgaris var. Black valentine, with particular reference to development of photosynthetic activity. Plant Physiol. 37, 473–480 (1962).
—: Effect of chloramphenicol on light dependent synthesis of proteins and enzymes of leaves and chloroplasts of Phaseolus vulgaris. Plant Physiol. 39, 579–585 (1964).
Müller, B.: On the mechanism of the light-induced activation of the NADP-dependent glyceraldehyde phosphate dehydrogenase. Biochim. biophys. Acta (Amst.) 205, 102–109 (1970).
— Ziegler, I., Ziegler, H.: Lichtinduzierte, reversible Aktivitätssteigerung der NADP-abhängigen Glycerinaldehyd-3-phosphat-Dehydrogenase in Chloroplasten. Europ. J. Biochem. 9, 101–106 (1969).
Wallis, M. E., Bradbeer, J. W.: Plastid development in primary leaves of Phaseolus vulgaris. Free nucleotide concentrations in greening leaves. J. exp. Bot. 21, 1039–1047 (1970).
Wildner, G. F., Criddle, R. S.: Ribulose diphosphate carboxylase. I. A factor involved in light activation of the enzyme. Biochem. biophys. Res. Commun. 37, 952–960 (1969).
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Ireland, H.M.M., Bradbeer, J.W. Plastid development in primary leaves of Phaseolus vulgaris . Planta 96, 254–261 (1971). https://doi.org/10.1007/BF00387444
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DOI: https://doi.org/10.1007/BF00387444