Summary
A soluble (supernatant) fraction from turnips catalyses the reduction of both FeCN and DCPIP but usually not cytochrome c in the presence of either NADH or NADPH. Slicing and aging turnip tissue induces an increase in these activities as well as the development of an NADH-cytochrome c reductase activity.
(NH4)2SO4 and Sephadex fractionation indicated that at least three enzymes were involved: an NADH-cytochrome-c reductase, an NADH-FeCN reductase, and an NAD(P)H-DCPIP and FeCN reductase. While the latter reductase had an acid pH optimum, indicating vacuolar origin, the NADH-cytochrome-c and FeCN reductases both had neutral pH optima, indicating cytoplasmic origin. Characterization of the NADH-specific reductases indicated that NADH-FeCN reductase may be a soluble form of the microsomal membrane NADH dehydrogenase but that NADH-cytochrome-c reductase may be normally soluble and possibly involved in cyanide-sensitive NADH oxidation.
The induced development of all three reductases was inhibited by 6-methylpurine, ethionine and cycloheximide, indicating dependence on both RNA and protein synthesis. The inhibition by cycloheximide could be reversed but this reversion required a 20-h washing-out period to be complete.
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Abbreviations
- DCPIP:
-
2,6-dichlorophenol indophenol
- FeCN:
-
ferricyanide
- NO QNO:
-
2-n-nonylhydroxyquinoline-N-oxide
- pCMB:
-
p-chloromercuribenzoate
- SF:
-
soluble fraction
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Rungie, J.M., Wiskich, J.T. Soluble electron-transport activities in fresh and aged turnip tissue. Planta 102, 190–205 (1972). https://doi.org/10.1007/BF00386890
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DOI: https://doi.org/10.1007/BF00386890