Summary
The secretion of the carbohydratous trapping slime of Drosophyllum is known to be mediated by the Golgi apparatus. In order to determine whether this secretion depends on the synthesis of new membrane material or runs for a long time by the cycling of membrane molecules, the influence of several inhibitors of protein synthesis was studied. Cycloheximide decreases the secretion of polysaccharides drastically, whereas puromycin and the amino acid analogues fluorophenylalanine and ethionine have little or no influence. An attempt is made to find explanations for these results, which, although contradictory, have parallels in the literature.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Flickinger, C.J.: Decreased formation of Golgi bodies in amebae in the presence of RNA and protein synthesis inhibitors. J. Cell Biol. 49, 221–226 (1971).
Goebel, K.: Pflanzenbiologische Schilderungen, Teil 2. Marburg: Elwert 1891–1893.
Hemmes, D.E., Hohl, H.R.: Ultrastructural aspects of encystation and cystgermination in Phytophthora parasitica. J. Cell Sci. 9, 175–191 (1971).
Herman, L., Fitzgerald, P.J.: The degenerative changes in pancreatic acinar cells caused by DL-ethionine. J. Cell Biol. 12, 277–296 (1962).
Hokin, L.E.: Dynamic aspects of phospholipids during protein secretion. Int. Rev. Cytol. 23, 187–208 (1968).
Jamieson, J.D., Palade, G.E.: Intracellular transport of secretory proteins in the pancreatic exocrine cell. III. Dissociation of intracellular transport from protein synthesis. J. Cell Biol. 39, 580–588 (1968).
Jurand, A., Jacob, J.: Electron microscope studies on salivary gland cells. VI. The effects of actinomycin D on the cytoplasm of Smittia parthenogenetica (Chironomidae). J. Insect Physiol. 11, 1345–1350 (1965).
Morré, D.J., In vivo incorporation of radioactive metabolites by Golgi apparatus and other cell fractions of onion stem. Plant Physiol. 45, 791–799 (1970).
Morré, D.J., Jones, D.D., Mollenhauer, H.H.: Golgi apparatus mediated polysaccharide secretion by outer root cap cells of Zea mays. I. Kinetics and secretory pathway. Planta (Berl.) 74, 286–301 (1967).
Nathans, D.: Puromycin inhibition of protein synthesis: incorporation of puromycin into peptid chains. Proc. nat. Acad. Sci. (N.Y.) 51, 585–592 (1964).
Schnepf, E.: Quantitative Zusammenhänge zwischen der Sekretion des Fangschleimes und den Golgi-Strukturen bei Drosophyllum lusitanicum. Z. Naturforsch. 16b, 605–610 (1961).
Schnepf, E.: Zur Cytologie und Physiologie pflanzlicher Drüsen. 1. Teil. Über den Fangschleim der Insektivoren. Flora 153, 1–22 (1963a).
Schnepf, E.: Zur Cytologie und Physiologie pflanlicher Drüsen. 3. Teil. Cytologische Veränderungen in den Drüsen von Drosophyllum während der Verdauung. Planta (Berl.) 59, 351–379 (1963b).
Schnepf, E.: Sekretion und Exkretion bei Pflanzen. Protoplasmatologia, VIII, 8. Wien-New York: Springer 1969.
Whaley, W.G., Dauwalder, M., Kephart, J.E.: The Golgi apparatus and an early stage in cell plate formation. J. Ultrastruct. Res. 15, 169–180 (1966).
Zaar, K., Schnepf, E.: Membranfluß und Nukleosiddiphosphatase-Reaktion in Wurzelhaaren von Lepidium sativum. Planta (Berl.) 88, 224–232 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schnepf, E. Über die Wirkung von Hemmstoffen der Proteinsynthese auf die Sekretion des Kohlenhydrat-Fangschleimes von Drosophyllum lusitanicum . Planta 103, 334–339 (1972). https://doi.org/10.1007/BF00386705
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00386705