Summary
Heat treatment of Phycomyces sporangiospores, which breaks dormancy, causes a very rapid 10- to 15fold increase in trehalase activity; soon after the heat shock the enzyme activity decays. This phenomenon can be repeated several times by repeating the heat shocks. Prolonging the heat treatment over the minimum required time delays the decay of enzyme activity. Cycloheximide does not prevent the rise in enzyme activity. It is suggested that heat treatment converts temporarily an inactive form of trehalase into an active one. Optimal enzyme activity is obtained at pH 7.5 and the enzyme requires metal ions for maximal activity. The possible role of trehalase in the spore-activation process is discussed.
Similar content being viewed by others
References
Borchert, R.: Über die Azetat-Aktivierung der Sporangiosporen von Phycomyces blakesleeanus. Beitr. Biol. Pflanzen 38, 31–61 (1962).
Halbsguth, W., Rudolph, H.: Untersuchungen über die Wärmeaktivierung der Sporangiosporen von Phycomyces blakesleeanus. I.. Arch. Mikrobiol. 32, 296–308 (1959).
Hill, E. P., Sussman, A. S.: Purification and properties of trehalase from Neurospora. Arch. Biochem. 102, 389–396 (1963).
Hill, E. P., Sussman, A. S.: Development of trehalase and invertase activity in Neurospora. J. Bact. 88, 1556–1566 (1964).
Horikoshi, K., Ikeda, Y.: Trehalase in conidia of Aspergillus oryzae. J. Bact. 91, 1883–1887 (1966).
Lowry, O. H., Rosebrough, J. F., Fan, A. L., Randall, J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Mandels, G. R., Vitols, R., Parrish, F. W.: Trehalose as an endogenous reserve in spores of the fungus Myrothecium verrucaria. J. Bact. 90, 1589–1598 (1965).
Rudolph, H.: Entwicklungsphysiologische Untersuchungen an den Sporangiophoren von Phycomyces blakesleeanus. Biol. Zbl. 77, 385–437 (1958).
Rudolph, H.: Weitere Untersuchungen zur Wärmeaktivierung der Sporangiosporen von Phycomyces blakesleeanus. Planta (Berl.) 54, 505–529 (1960).
Rudolph, H.: Weitere Untersuchungen zur Wärmeaktivierung der Sporangiosporen von Phycomyces blakesleeanus. III. Planta (Berl.) 57, 284–312 (1961).
Rudolph, H., Furch, B.: Untersuchungen zur Aktivierung von Sporenhomogenaten durch Wärmebehandlung. Arch. Mikrobiol. 72, 175–181 (1970).
Rudolph, H., Ochsen, B.: Trehalose-Umsatz wärmeaktivierter Sporen von Phycomyces blakesleeanus. Arch. Mikrobiol. 65, 163–171 (1969).
Sinohara, H.: Induction of enzymes in dormant spores of Aspergillus oryzae. J. Bact. 101, 1070–1072 (1970).
Sussman, A. S.: The prevalence and role of dormancy. In: The bacterial spore (G. W. Gould and A. Hurst, eds.), p. 1–38. New York: Acad. Press 1969.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Van Assche, J.A., Carlier, A.R. & Dekeersmaeker, H.I. Trehalase activity in dormant and activated spores of Phycomyces blakesleeanus . Planta 103, 327–333 (1972). https://doi.org/10.1007/BF00386704
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00386704