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Über die Regulation der Glucose-6-phosphat-Dehydrogenase in Spinatchloroplasten durch Licht

Regulation of glucose-6-phosphate dehydrogenase in spinach chloroplasts by light

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Summary

  1. 1.

    The activity of the glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADPH oxidoreductase, EC 1.1.1.49) in the homogenate of illuminated leaves of Spinacea oleracea L. (30 min with 30000 Lux) is about 30% lower than in the dark controls.

  2. 2.

    Illumination of isolated chloroplasts (10000 Lux) causes a fast inhibition of the enzyme activity which reaches about 45% after 20 min.

  3. 3.

    The activity of the 6-phosphogluconate dehydrogenase (6-phospho D-gluconate: NADP+ oxidoreductase, EC 1.1.1.44) is not affected by illumination.

  4. 4.

    These results confirm the idea that illumination results in a switch from the oxidative to the reductive pentose phosphate cycle via light-dependent regulation of glucose-6-phosphate dehydrogenase.

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Abbreviations

ATP:

Adenosintriphosphat

G-6-P:

Glucose-6-phosphat

G-6-P-DH:

Glucose-6-phosphat-Dehydrogenase

HEPES:

N-2-Hydroxyäthylpiperazin-N′-2-äthansulfonsäure

MES:

2(N-morpholino)-äthansulfonsäure

NAD+ :

oxydiertes Nicotinamid-adenin-dinukleotid

NADP+ :

oxydiertes Nicotinamid-adenin-dinukleotid-phosphat

NADH (für NADH+H+):

reduziertes Nicotinamid-adenin-dinukleotid

NADPH (für NADH+H+):

reduziertes Nicotinamid-adenindinukleotid-phosphat

6-P-G:

6-Phospho-gluconsäure

6-P-G-DH:

6-Phosphogluconsäure-Dehydrogenase

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Authors and Affiliations

  1. Botanisches Institut der Technischen Hochschule Darmstadt, Darmstadt, Deutschland

    K. Lendzian & H. Ziegler

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  1. K. Lendzian
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  2. H. Ziegler
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Lendzian, K., Ziegler, H. Über die Regulation der Glucose-6-phosphat-Dehydrogenase in Spinatchloroplasten durch Licht. Planta 94, 27–36 (1970). https://doi.org/10.1007/BF00386606

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  • DOI: https://doi.org/10.1007/BF00386606

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