Summary
Irradiation of homogenates of etiolated barley leaves with red light resulted in an increase in the levels of gibberellin (GA)-like substances as compared to dark controls. When homogenates were fed with [3H]-GA9 there was as incorporation of the radioactivity into a number of other GA's: this process occurred to a greater extent in red light than in darkness, and could be inhibited by boiling the extract prior to addition of the [3H]-GA9.
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Barendse, G. W. M., Kok, N. J. J.: Incorporation of 14C-kaurene into the gibberellin of a higher plant (Pharbitis nil Chois). Plant Physiol. 48, 476–479 (1971).
Barendse, G. W. M., Kende, H., Lang, A.: Fate of radioactive gibberellin A1 in maturing and germinating seeds of pea and Japanese morning glory. Plant Physiol. 43, 815–822 (1968).
Bassham, J. A., Jensen, R. G.: In: Harvesting the sun, San Pietro, A., Green, F. A., and Army, T. J., eds., p. 79–100. New York-London: Acad. Press 1967.
Beevers, L., Loveys, B., Pearson, J. A., Wareing, P. F.: Phytochrome and hormonal control of expansion and greening of etiolated wheat leaves. Planta (Berl.) 90, 80–86 (1970).
Brian, P. W., Hemming, H. G., Lowe, D.: Comparative potency of nine gibberellins. Ann. Bot. 28, 369–389 (1964).
Cross, B. E., Norton, K., Stewart, J. C.: The biosynthesis of the gibberellins. III. J. chem. Soc. C, 1054–1063 (1968).
Ende, H. van den, Zeevaart, J. A. D.: Influence of daylength on gibberellin metabolism and stem growth in Silene armeria. Planta (Berl.) 98, 164–176 (1971).
Geissman, T. A., Verbiscar, A. J., Phinney, B. O., Cragg, G.: Studies on the biosynthesis of gibberellins from (-)-kaurenoic acid in cultures of Gibberellia fujikuroi. Phytochem. 5, 933–947 (1966).
Graebe, J. E., Bowen, D. H., MacMillan, J.: The conversion of mevalonic acid into gibberellin A12 aldehyde in a cell free system from Cucurbita pepo. Planta (Berl.) 102, 261–271 (1972).
Hedley, C. L., Stoddart, J. L.: Light-stimulated of alanine amino-transferase activity in dark-grown leaves of Lolium temulentum L. as related to chlorophyll formation. Planta (Berl.) 100, 309–324 (1971).
Jones, R. L.: Agar diffusion techniques for estimating gibberellin production by plant organs; the discrepancy between extractable and diffusible gibberellins in pea. In: Biochemistry and physiology of plant growth substances, F. Wightman and G. Setterfield, eds., p. 73–84. Ottawa: Runge Press 1968.
Jones, R. L., Stoddart, J. L., MacMillan, J., Cloke, C.: Structural modification of gibberellins by in vitro hydroxylating systems. Planta (Berl.) 101, 231–241 (1971).
Katsumi, M., Phinney, B. O.: Biosynthesis and metabolism of gibberellins. In: Gibberellins, Tamura, S., ed., p. 195–219. Tokyo: University Press 1969.
Kende, M.: Preparation of radioactive gibberellin A1 and its metabolism in dwarf peas. Plant Physiol. 42, 1612–1618 (1967).
Lew, F. T., West, C. A.: (-)-Kaur-16-en-7β-ol-19-oic acid, an intermediate in gibberellin biosynthesis. Phytochem. 10, 2065–2070 (1972).
Loveys, B. R., Wareing, P. F.: The red light controlled production of gibberellin in etiolated wheat leaves. Planta (Berl.) 98, 109–116 (1971).
Müller, B., Ziegler, H.: Die lichtinduzierte Aktivitätssteigerung der NADP+-abhängigen Glycerinaldehyd-3-phosphate. Planta (Berl.) 85, 96–104 (1969).
Railton, I. D., Wareing, P. F.: Effects of daylength on endogenous gibberellins in Solanum andigena. III. Gibberellin production by the leaves. In prep
Reid, D. M., Clements, J. B.: RNA and protein synthesis; prerequisities of red light-induced gibberellin synthesis. Nature (Lond.) 219, 607–609 (1968).
Reid, D. M., Clements, J. B., Carr, D. J.: Red light induction of gibberellin synthesis in leaves. Nature (Lond.) 217, 580–582 (1968).
Reid, D. M., Crozier, A.: CCC-induced increase of gibberellin levels in pea seedlings. Planta (Berl.) 94, 95–106 (1970).
Sembdner, G., Weiland, J., Aurich, O., Schreiber, K.: Isolation, structure and metabolism of a gibberellin glucoside. In: Plant growth regulators, S.C.I. Monogr. No. 31, 70–86 (1968).
Stoddart, J. L., Lang, A.: The effect of daylength on gibberellin synthesis in leaves of red clover (Trifolium pratense L.). In: Biochemistry and physiology of plant growth substances, F. Wightman and G. Setterfield, eds., p. 1371–1383. Ottawa: Runge Press 1968.
Verbiscar, A. J., Cragg, F., Geissman, T. A., Phinney, B. O.: Studies on the biosynthesis of gibberellins. II. Phytochem. 6, 807–814 (1967).
Wildner, G. F., Criddle, R. S.: Ribulose diphosphate carboxylase I. A factor involved in light activation of enzyme. Biochem. biophys. Res. Commun. 37, 952–960 (1969).
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Supported by National Research Council (Canada) grant A-5727 (Dr. D. M. Reid).
Supported by National Research Council (Canada) grant A-2585 (Dr. R. P. Pharis).
Supported by NATO-Science Research Council (U. K.) Postdoctoral Fellowship.
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Reid, D.M., Tuing, M.S., Durley, R.C. et al. Red-light-enhanced conversion of tritiated gibberellin A9 into other gibberellin-like substances in homogenates of etiolated barley leaves. Planta 108, 67–75 (1972). https://doi.org/10.1007/BF00386507
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DOI: https://doi.org/10.1007/BF00386507