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Variation in the nitrate reductase of rice seedlings

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Summary

Nitrate reductase was induced in rice seedlings by nitrate and by chloramphenicol. During the induction period the different enzyme activities associated with nitrate reductase increased to different degrees. Nitrate induced high NADH-nitrate reductase activity and a great increase in the NADH-cytochrome c reductase activity which was associated with the nitrate reductase in a sucrose gradient. Chloramphenicol induced a nitrate reductase which had higher activity with NADPH than NADH. Chloramphenicol also induced a marked increase in NADPH-cytochrome c reductase activity as well as in NADH-cytochrome c reductase activity. Both activities were associated with the nitrate reductase in a sucrose gradient.

After partial purification by sucrose gradient sedimentation or by starch gel electrophoresis, the nitrate reductase of rice induced by nitrate and chloramphenicol showed the same preference in pyridine nucleotide cofactors as was shown by the crude enzyme extracts.

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References

  • Beevers, L., Flesher, D., Hageman, R. H.: Studies on the pyridine nucleotide specificity of nitrate reductase in higher plants and its relationship to sulfhydryl level. Biochim. biophys. Acta (Amst.) 89, 453–464 (1964).

    Google Scholar 

  • Garrett, R. H., Nason, A.: Further purification and properties of Neurospora nitrate reductase. J. biol. Chem. 244, 2870–2882 (1969).

    Google Scholar 

  • Martin, R. G., Ames, B. N.: A method for determining the sedimentation behavior of enzymes: Application to protein mixtures. J. biol. Chem. 236, 1372–1379 (1961).

    PubMed  Google Scholar 

  • Notton, B. A., Hewitt, E. J.: The role of tungsten in the inhibition of nitrate reductase activity in spinach (Spinacea oleracea L.) leaves. Biochem. biophys. Res. Commun. 44, 702–710 (1971).

    Google Scholar 

  • Scandalios, J. G.: The genetic control of multiple molecular forms of enzymes in plants: a review. Biochem. Genet. 3, 37–79 (1969).

    Google Scholar 

  • Shen, T. C.: The induction of nitrate reductase and the preferential assimilation of ammonium in germinating rice seedlings. Plant Physiol. 44, 1650–1655 (1969).

    Google Scholar 

  • Shen, T. C.: Nitrate reductase of rice seedlings and its induction by organic nitrocompounds. Plant Physiol. 49, 546–549 (1972).

    Google Scholar 

  • Sims, A. P., Folkes, B. F., Bussey, A. H.: Mechanisms involved in the regulation of nitrogen assimilation in micro-organisms and plants. In: Recent aspect of nitrogen metabolism in plants, p. 91–114, Hewitt, E. J., Cutting, C. V., eds. New York: Acad. Press 1968.

    Google Scholar 

  • Sorger, G. J.: Nitrate reductase electron transport systems in mutant and in wildtype strains of Neurospora. Biochim. biophys. Acta (Amst.) 118, 484–494 (1966).

    Google Scholar 

  • van'T, Riet, J., Stouthamer, A. H., Planta, R. J.: Regulation of nitrate assimilation and nitrate respiration in Aerobacter aerogenes. J. Bact. 96, 1455–1464 (1968).

    PubMed  Google Scholar 

  • Wray, J. L., Filner, P.: Structural and functional relationships of enzyme activities induced by nitrate in barley. Biochem. J. 119, 715–725 (1970).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. MSU/AEC Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA

    T. C. Shen

  2. School of Biological Sciences, University of Malaya, Pantai Valley, Kuala Lumpur, Malaysia

    T. C. Shen

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  1. T. C. Shen
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Shen, T.C. Variation in the nitrate reductase of rice seedlings. Planta 108, 21–28 (1972). https://doi.org/10.1007/BF00386503

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  • DOI: https://doi.org/10.1007/BF00386503

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