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Localization and uptake of 14C-IAA in relation to xylem regeneration in Coleus internodes

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Summary

14C-IAA was applied to decapitated Coleus 5th internodes. Sections were radioautographed at various intervals after the application. The location of the label within cells and tissues is discussed. No accumulation of label occurred in the region where xylem cells regenerated. Label was particularly associated with xylem and phloem cytoplasm, but also occurred in other cells.

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References

  • Fosket, D. E.: Celldivision and the differentiation of wound-vessel members in cultured stem segments of Coleus. Proc. nat. Acad. Sci. (Wash.) 59, 1089–1096 (1968).

    Google Scholar 

  • Gifford, E. M. Jr., Nitsch, J. P.: Responses of tobacco pith nuclei to growth substances. Planta (Berl) 85, 1–10 (1969).

    Google Scholar 

  • Jacobs, W. P.: Acropetal aucin transport and xylem regeneration—a quantitative study. Amer. Naturalist 842, 327–337 (1954).

    Article  Google Scholar 

  • Jacobs, W. P.: Comparison of the movement and vascular differentiation effects of the endogenous auxin and of phenoxyacetic weedkillers in stems and petioles of Coleus and Phaseolus. Ann. N.Y. Acad. Sci. 144, 102–117 (1967).

    Google Scholar 

  • Jacobs, W. P., McCready, C. C.: Polar transport of growth regulators in pith and vascular tissue of Coleus stems. Amer. J. Bot. 54, 1035–1040 (1967).

    Google Scholar 

  • Robinson, B. J., Foreman, M., Addicote, F. T.: Auxin transport and conjugation in cotton explants. Plant Physiol. 43, 1321–1323 (1968).

    Google Scholar 

  • Sabnis, D. D., Hirschberg, G., Jacobs, W. P.: Radioautographic analysis of the distribution of label from 3H-indoleacetic acid supplied to isolated Coleus internodes. Plant Physiol. 44, 27–36 (1969).

    Google Scholar 

  • Sachs, T.: Polarity and the induction of organised vascular tissue. Ann. Bot. 33, 263–275 (1969).

    Google Scholar 

  • Wangermann, E.: Autoradiographic localization of soluble and insoluble 14C from 14C indoleacetic acid supplied to isolated Coleus internodes. New Phytol. 69, 919–927 (1970).

    Google Scholar 

  • Zwar, J. A., Brown, R.: Distribution of labelled plant growth regulators within cells. Nature 220, 500–501 (1968).

    PubMed  Google Scholar 

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This work was supported by the Canadian Research Council. We thank Dr. R. B. van Huystee for advice on radioautography and Mrs. L. McKay for advice on microtechnique.

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Gee, H. Localization and uptake of 14C-IAA in relation to xylem regeneration in Coleus internodes. Planta 108, 1–9 (1972). https://doi.org/10.1007/BF00386501

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  • DOI: https://doi.org/10.1007/BF00386501

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