Abstract
Four photosynthetic bacteria, isolated from 14 samples taken from seafood processing plants, were identified as species of Rhodocyclus gelatinosus, belonging to the purple, non-sulphur bacteria of the family Rhodospirillaceae. Cultivation in synthetic medium under four different conditions indicated that all four strains gave maximum carotenoid and bacteriochlorophyll synthesis under anaerobic conditions in the light, with values of 11 to 12.6 and 102 to 108 mg/g dry cell wt, respectively. These values are 87% higher than the pigment content obtained from aerobic cultivation, although the cell biomass of all strains (1.7 to 2.3 g/l) was 22 to 38% higher under aerobic conditions. Protein content was always between 32 and 43%. The specific growth rates of all isolates in aerobic cultivation (0.04 to 0.06 h-1) were twice those in anaerobic conditions in the light. No growth occurred in anaerobic conditions in the dark.
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References
American Public Health Association 1985 Standard Methods for the Examination of Water and Wastewater, 16th edn. Washington DC: APHA.
Association of Official Analytical Chemists 1984 Official Methods of Analysis of the Association of Official Chemists, 14th edn. Washington DC: AOAC.
Buchanan, R.C., Gibbons, N.E., Cowan, S.T., Holt, J.G., Liston, J., Murray, R.G.E. & Stanier, R.Y. (eds) 1974 Bergey's Manual of Determinative Bacteriology, 8th edn. Baltimore: Williams & Wilkins.
Cohen-Bazire, G., Sistrom, W.R. & Stanier, R.Y. 1957 Kinetic studies of pigment synthesis by non-sulfur purple bacteria. Journal of Cellular and Comparative Physiology 49, 25–68.
Devlin, R.M. & Barker, A.V. 1971 Photosynthesis. New Delhi: Affiliated East-West Press.
Drews, G. 1981 Rhodospirillum salexigens, spec. nov., obligatory halophilic phototrophic bacteria. Archives of Microbiology 130, 325–327.
Hirayama, O. 1968 Lipids and lipoprotein complex in photosynthetic tissue: 4 lipid and pigments of photosynthetic bacteria. Agricultural and Biological Chemistry 32, 34–41.
Imhoff, J.F. 1988 Anoxygenic phototrophic bacteria. In Methods in Aquatic Bacteriology, ed Austin, B. pp. 207–240. New York: Wiley Interscience.
Imhoff, J.F., Truper, H.G. & Pfennig, N. 1984 Rearrangement of the species and genera of the photosynthetic purple nonsulfur bacteria. Journal of Systematic Bacteriology 34, 340–343.
Kobayashi, M. & Kurata, S. 1978 The mass culture and cell utilization of photosynthetic bacteria. Process Biochemistry 13, 27–30.
Kohlmiler, E.F. & Gest, H. 1951 A comparative study fermentations of organic acids by Rhodospirillum rubrum. Journal of Bacteriology 61, 269–282.
Noparatnaraporn, N. & Nagai, S. 1986 Selection of Rhodobacter sphaeroides P47 as a useful source of single cell protein. Journal of General and Applied Microbiology 35, 351–359.
Pellerin, N.B. & Gest, M. 1983 Diagnostic features of the photosynthetic bacterium Rhodopseudomonas sphaeroides. Current Microbiology 9, 339–344.
Pfennig, N. 1967 Photosynthetic bacteria. Annual Review of Microbiology 21, 285–324.
Pfennig, N. & Truper, H.G. 1974 The photosynthetic bacteria. In Bergey's Manual of Determinative Bacteriology, 8th edn, eds Buchanan, R.C., Gibbons, N.E., Cowan, S.T., Holt, J.G., Liston, J., Murray, R.G.E. & Stanier, R.Y. pp. 24–75. Baltimore: Williams & Wilkins.
Prasertsan, P. & Choorit, W. 1988 Problem and solution of the occurrence of red color in wastewater of seafood processing plant. Songklanakarin Journal of Science and Technology 10, 439–466.
Shipman, R.N., Fan, L.T. & Kao, I.C. 1977 Single cell protein production by photosynthetic bacteria. Advances in Applied Microbiology 21, 161–181.
Staley, J.T., Bryant, M.P., Pfennig, N. & Holt, J.G. 1989 Bergey's Manual of Systematic Bacteriology, 3rd edn, pp. 1678–1682. Baltimore: Williams & Wilkins.
Vrati, S. 1984 Single cell protein production by photosynthetic bacteria grown on the clarified effluents of biogas plants. Applied Microbiology and Biotechnology 19, 199–202.
Watanabe, K., Kim, J.S., Ito, K., Buranarkarl, L., Kampee, T. & Takashi, H. 1981 Thermostable nature of hydrogen production by non-sulfur purple photosynthetic bacteria isolated in Thailand. Agricultural and Biological Chemistry 45, 217–222.
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Prasertsan, P., Choorit, W. & Suwanno, S. Isolation, identification and growth conditions of photosynthetic bacteria found in seafood processing wastewater. World J Microbiol Biotechnol 9, 590–592 (1993). https://doi.org/10.1007/BF00386301
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DOI: https://doi.org/10.1007/BF00386301