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Biomass and pigments production of a newly isolated photosynthetic bacterium Ectothiorhodospira shaposhnikovii P2 from saline wastewater

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Abstract

Saline wastewater generation has increased in recent years, and saline-tolerant bacteria are very important for the treatment of saline wastewater. In this study, a haloduric photosynthetic bacterium Ectothiorhodospira shaposhnikovii P2 was isolated, and its cell biomass, pigments accumulation, and pollutants removal capacity were analyzed during salinity wastewater treatment process. The results showed that the optimal pH for obtaining maximum cell biomass (1.161 ± 0.021 g/L) and COD (95.83 ± 0.32%) and NH4+–N (87.72 ± 0.88%) removal was 8.0, whereas that for achieving high carotenoids (17.3 ± 0.2 mg/L) and bacteriochlorophylls (52.2 ± 2.3 mg/L) yields was 8.5. Thus, wastewater treatment processes using photosynthetic bacteria have the advantage of accumulating valuable biomass together with pollutant removal.

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References

  • Bong KM, Kim KM, Seo MK, Han JH, Park IC, Lee CW, Kim PI, Bong KM, Kim KM, Seo MK (2017) Optimization of medium for the carotenoid production by Rhodobacter sphaeroides PS-24 using response surface methodology. Korean J Org Agric 25:135–148

  • Bryantseva IA, Tourova TP, Kovaleva OL, Kostrikina NA, Gorlenko VM (2010) Ectothiorhodospira magna sp. nov., a new large alkaliphilic purple sulfur bacterium. Microbiology 79:780–790

    Article  CAS  Google Scholar 

  • Cai J, Wang G (2012) Hydrogen production by a marine photosynthetic bacterium, Rhodovulum sulfidophilum P5, isolated from a shrimp pond. Int J Hydrog Energy 37:15070–15080

    Article  CAS  Google Scholar 

  • Federation WE, APH Association (2005) Standard methods for the examination of water and wastewater. American Public Health Association (APHA), Washington

    Google Scholar 

  • Gui M, Chen Q, Ni J (2017) Effect of NaCl on aerobic denitrification by strain Achromobacter sp. GAD-3. Appl Microbiol Biotechnol 101(12):5139–5147

    Article  CAS  Google Scholar 

  • Han H, Jia Q, Liu B, Yang H, Shen J (2013) Fermentative hydrogen production from acetate using Rhodobacter sphaeroides RV. Int J Hydrog Energy 38:10773–10778

    Article  CAS  Google Scholar 

  • Imhoff JF (2015) Ectothiorhodospira, Bergey’s manual of systematics of Archaea and Bacteria. Wiley, New York

    Google Scholar 

  • Imhoff JF, Trüper HG (1977) Ectothiorhodospira halochloris sp. nov., a new extremely halophilic phototrophic bacterium containing bacteriochlorophyll b. Arch Microbiol 114:115–121

    Article  CAS  Google Scholar 

  • Kim MK, Choi K-M, Yin C-R, Lee K-Y, Im W-T, Lim JH, Lee S-T (2004) Odorous swine wastewater treatment by purple non-sulfur bacteria, Rhodopseudomonas palustris, isolated from eutrophicated ponds. Biotechnol Lett 26:819–822

    Article  CAS  Google Scholar 

  • Kompantseva EI, Komova AV, Novikov AA, Kostrikina NA (2012) Rhodovulum tesquicola sp. nov., a haloalkaliphilic purple non-sulfur bacterium from brackish steppe soda lakes. Int J Syst Evol Microbiol 62:2962–2966

    Article  CAS  Google Scholar 

  • Liu S, Zhang G, Zhang J, Li X, Li J (2016) Performance, carotenoids yield and microbial population dynamics in a photobioreactor system treating acidic wastewater: effect of hydraulic retention time (HRT) and organic loading rate (OLR). Bioresour Technol 200:245–252

    Article  CAS  Google Scholar 

  • Lu H, Zhang G, Wan T, Lu Y (2011) Influences of light and oxygen conditions on photosynthetic bacteria macromolecule degradation: different metabolic pathways. Bioresour Technol 102:9503–9508

    Article  CAS  Google Scholar 

  • McWilliiams A (2018) BBC Research: the global market for carotenoids. https://www.bccresearch.com/market-research/food-and-beverage/the-global-market-for-carotenoids-fod025f.html. Accessed June 2018

  • Meng F, Yang A, Zhang G, Wang H (2017) Effects of dissolved oxygen concentration on photosynthetic bacteria wastewater treatment: pollutants removal, cell growth and pigments production. Bioresour Technol 241:993–997

    Article  CAS  Google Scholar 

  • Meng F, Yang A, Wang H, Zhang G, Li X, Zhang Y, Zou Z (2018) One-step treatment and resource recovery of high-concentration non-toxic organic wastewater by photosynthetic bacteria. Bioresour Technol 251:121–127

    Article  CAS  Google Scholar 

  • Mezzomo N, Ferreira SRS (2016) Carotenoids functionality, sources, and processing by supercritical technology: a review. J Chem 2016:1–16. https://doi.org/10.1155/2016/3164312

    Article  Google Scholar 

  • Pantiushenko IV, Rudakovskaya PG, Starovoytova AV, Mikhaylovskaya AA, Abakumov MA, Kaplan MA, Tsygankov AA, Majouga AG, Grin MA, Mironov AF (2015) Development of bacteriochlorophyll a-based near-infrared photosensitizers conjugated to gold nanoparticles for photodynamic therapy of cancer. Biochemistry-Moscow 80:752–762

    Article  CAS  Google Scholar 

  • Roohbakhsh A, Karimi G, Iranshahi M (2017) Carotenoids in the treatment of diabetes mellitus and its complications: a mechanistic review. Biomed Pharmacother 91:31–42

    Article  CAS  Google Scholar 

  • Saejung C, Ampornpat W (2017) Production and nutritional performance of carotenoid-producing photosynthetic bacterium Rhodopseudomonas faecalis PA2 grown in domestic wastewater intended for animal feed production. Waste Biomass Valoriz 1–12

  • Saejung C, Apaiwong P (2015) Enhancement of carotenoid production in the new carotenoid-producing photosynthetic bacterium Rhodopseudomonas faecalis PA2. Biotechnol Bioprocess Eng 20:701–707

    Article  CAS  Google Scholar 

  • Saejung C, Thammaratana T (2016) Biomass recovery during municipal wastewater treatment using photosynthetic bacteria and prospect of production of single cell protein for feedstuff. Environ Technol 37:3055–3061

    Article  CAS  Google Scholar 

  • Sowmya R, Sachindra NM (2015) Carotenoid production by Formosa sp. KMW, a marine bacteria of Flavobacteriaceae family: influence of culture conditions and nutrient composition. Biocatal Agric Biotechnol 4:559–567

    Article  Google Scholar 

  • Sui L, Liu L, Deng Y (2014) Characterization of halophilic C-50 carotenoid-producing archaea isolated from solar saltworks in Bohai Bay, China. Chin J Oceanol Limnol 32:1280–1287

    Article  CAS  Google Scholar 

  • Talaiekhozani A, Rezania S (2017) Application of photosynthetic bacteria for removal of heavy metals, macro-pollutants and dye from wastewater: a review. J Water Process Eng 19:312–321

    Article  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  Google Scholar 

  • Uygur A (2006) Specific nutrient removal rates in saline wastewater treatment using sequencing batch reactor. Process Biochem 41:61–66

    Article  CAS  Google Scholar 

  • Wang H, Yang A, Zhang G, Ma B, Meng F, Peng M, Wang H (2017a) Enhancement of carotenoid and bacteriochlorophyll by high salinity stress in photosynthetic bacteria. Int Biodeterior Biodegrad 121:91–96

    Article  CAS  Google Scholar 

  • Wang JL, Gong BZ, Huang W, Wang YM, Zhou J (2017b) Bacterial community structure in simultaneous nitrification, denitrification and organic matter removal process treating saline mustard tuber wastewater as revealed by 16S rRNA sequencing. Bioresour Technol 228:31–38

    Article  CAS  Google Scholar 

  • Watanabe M, Sasaki K, Nakashimada Y, Kakizono T, Noparatnaraporn N, Nishio N (1998) Growth and flocculation of a marine photosynthetic bacterium Rhodovulum sp. Appl Microbiol Biotechnol 50:682–691

    Article  CAS  Google Scholar 

  • Wen S, Liu H, He H, Luo L, Li X, Zeng G, Zhou Z, Lou W, Yang C (2016) Treatment of anaerobically digested swine wastewater by Rhodobacter blasticus and Rhodobacter capsulatus. Bioresour Technol 222:33–38

    Article  CAS  Google Scholar 

  • Xavier AAO, Perez-Galvez A (2016) Carotenoids as a source of antioxidants in the diet. Sub-cell Biochem 79:359–375

    Article  CAS  Google Scholar 

  • Zagrodnik R, Laniecki M (2017) The effect of pH on cooperation between dark-and photo-fermentative bacteria in a co-culture process for hydrogen production from starch. Int J Hydrog Energy 42:2878–2888

    Article  CAS  Google Scholar 

  • Zhang H, Hu Q-P (2015) Isolation, identification and physiological characteristics of high carotenoids yield Rhodopseudomonas faecalis PSB-B. Int J Recent Sci Res 6:3893–3899

    Google Scholar 

  • Zhang X, Wu Y, Xiao G, Tang Z, Wang M, Liu F, Zhu X (2017) Simultaneous photocatalytic and microbial degradation of dye-containing wastewater by a novel g-C3N4-P-25/photosynthetic bacteria composite. PLoS ONE 12:e0172747

    Article  Google Scholar 

  • Zhou Q, Zhang P, Zhang G (2014) Biomass and carotenoid production in photosynthetic bacteria wastewater treatment: effects of light intensity. Bioresour Technol 171:330–335

    Article  CAS  Google Scholar 

  • Zhou Q, Zhang PY, Zhang GM (2015a) Biomass and pigments production in photosynthetic bacteria wastewater treatment: effects of light sources. Bioresour Technol 179:505–509

    Article  CAS  Google Scholar 

  • Zhou Q, Zhang PY, Zhang GM, Peng M (2015b) Biomass and pigments production in photosynthetic bacteria wastewater treatment: effects of photoperiod. Bioresour Technol 190:196–200

    Article  CAS  Google Scholar 

  • Zwietering MH, Jongenburger I, Rombouts FM, van’t Riet K (1990) Modeling of the bacterial growth curve. Appl Environ Microbiol 56:1875–1881

    CAS  Google Scholar 

Download references

Acknowledgements

This work was support by the Natural Science Foundation of Tianjin of China (17JCYBJC22900), Student Laboratory Innovation Fund of Tianjin University of Science and Technology (1803A203) and Qingdao Science and Technology People-Benifit Project (18-6-1-116-nsh).

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Authors and Affiliations

  1. Tianjin Key Laboratory of Marine Resources and Chemistry, College of Chemistry Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin, 300457, China

    J. Cai, Y. Guan, Y. Zhao, C. Feng & N. Tang

  2. Tianjin International Joint Academy of Biomedicine, Tianjin, 300457, China

    J. Cai

  3. Qingdao University of Science and Technology, Qingdao, 266042, China

    F. Li

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  1. J. Cai
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  2. Y. Guan
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  3. F. Li
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  4. Y. Zhao
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  5. C. Feng
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  6. N. Tang
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Correspondence to J. Cai.

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Editorial responsibility: M. Abbaspour.

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Cai, J., Guan, Y., Li, F. et al. Biomass and pigments production of a newly isolated photosynthetic bacterium Ectothiorhodospira shaposhnikovii P2 from saline wastewater. Int. J. Environ. Sci. Technol. 16, 7487–7496 (2019). https://doi.org/10.1007/s13762-018-2141-9

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  • DOI: https://doi.org/10.1007/s13762-018-2141-9

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