Summary
Under most culture conditions only 0.3% of the vegetative spores of Phycomyces blakesleeanus are uninucleate. On an acidified minimal medium, the uninucleate fraction can be raised up to 4.5% of the spores. The spore population can be fractionated in a gradient under gravity (1xg) yielding fractions that contain over 80% uninucleate spores. These uninucleate spores are fully viable. When the spores to be fractionated are obtained from a heterokaryotic mycelium, the uninucleate fraction produces homokaryotic mycelia.
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Reau, P. Uninucleate spores of Phycomyces . Planta 108, 153–160 (1972). https://doi.org/10.1007/BF00386077
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DOI: https://doi.org/10.1007/BF00386077