Abstract
The expression of genes in particular for light-harvesting chlorophyll a/b protein (LHCP) and ribulose-1,5-bisphosphate carboxylase (RuBPCase) has been studied in the developing barley leaf. This has been done by analysis of the occurrence of both proteins within the different regions (1 to 6, beginning from the base) of the primary 7-day-old leaf. It has been found that LHCP already appears in the base of the leaf, whereas RuBPCase is primarily expressed in the apical expanding part of the leaf. The distribution of the mRNAs for both proteins within this gradient is in accordance with that of the proteins themselves, indicating that gene expression is not regulated at the level of translation in both cases. The poly(A) mRNA for LHCP occurs mainly in the basic sections 2 and 3, whereas that for RuBPCase is found throughout the leaf but primarily in the apical sections of the leaf.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- LHCP:
-
light-harvesting chlorophyll a/b protein
- RuBPCase:
-
ribulose-1,5-bisphosphate carboxylase
- TCA:
-
trichloroacetic acid
References
Apel, K., Kloppstech, K. (1978) The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein. Eur. J. Biochem. 85, 581–588
Armond, P.A., Staehelin, L.A., Arntzen, C.J. (1977) Spatial relationship of photosystem I, photosystem II and the light-harvesting complex in chlorplast membranes. J. Cell Biol. 73, 400–418
Arnon, D.J. (1949) Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15
Baker, N.R., Leech, R.M. (1977) Development of photosystem I and photosystem II activities in leaves of light-grown maize (Zea mays). Plant Physiol. 60, 640–644
Boffey, S.A., Selldén, G., Leech, R.M. (1980) The influence of cell age on chlorophyll formation in light-grown and etiolated wheat seedlings. Plant Physiol. 65, 680–684
Bonner, W.M., Laskey, R.A. (1974) A film detection method for tritium-labelled proteins. Eur. J. Biochem. 46, 83–88
Chua, N.H., Schmidt, G.W. (1978) Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc. Nat. Acad. Sci. USA 75, 6110–6114
Chua, N.H., Schmidt, G.W. (1979) Transport of proteins into mitochondria and chloroplasts. J. Cell Biol. 81, 461–483
Ellis, R.J., Bogorad, L. (1971) A one-step method for the isolation and determination of leaf ribulose-1,5-diphosphate carboxylase. Anal. Biochem. 41, 57–66
Heber, U., Krause, G.H. (1980) What is the physiological role of photorespiration? TIBS 5, 32–34
Kloppstech, K., Schweiger, H.G. (1976) In vitro translation of poly(A)RNA from Acetabularia. Cytobiologie 13, 394–400
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275
Müller, M., Viro, M., Balke, Ch., Kloppstech, K. (1980) Kinetics of appearance of mRNA for light-harvesting chlorophyll a/b protein in polysomes of barley. Planta 148, 448–452
Robertson, D., Laetsch, W. McM. (1974) Structure and function of developing barley plastids. Plant Physiol. 54, 148–159
Viro, M., Kloppstech, K., Apel, K., Schweiger, H.G. (in preparation) Two precursor molecules for the light-harvesting chlorophyll a/b complex obtained in vitro by translation of poly(A) RNA from Acetabularia
Weinbaum, S.A., Gressel, J., Reisfeld, A., Edelman, M. (1979) Characterization of the 32.000 dalton chloroplast membrane protein. III. Probing its biological function in Spirodela. Plant Physiol 64, 828–832
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Viro, M., Kloppstech, K. Differential expression of the genes for ribulose-1,5-bisphosphate carboxylase and light-harvesting chlorophyll a/b protein in the developing barley leaf. Planta 150, 41–45 (1980). https://doi.org/10.1007/BF00385613
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00385613