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Ca2+ transport in mitochondrial and microsomal fractions from higher plants

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Abstract

Mitochondria from etiolated corn possess a much greater Ca2+ uptake capacity per mg protein than microsomes from the same source. Differences in energy requirements, sensitivity to specific inhibitors, and sedimentation properties enabled us to study both Ca2+ uptake mechanisms without mutual contamination. The microsomal Ca2+ uptake does not vary much among different plants as compared to the mitochondrial Ca2+ uptake; this is also true for different organs of the same plant. Mitochondrial Ca2+ uptake is more dependent on the age of the seedlings than microsomal uptake, because of changes in active Ca2+ uptake activity rather than of changes in efflux. Intactness and the oxidative and phosphorylative properties of the mitochondria remained unchanged during this time period. Na+ and Mg2+ do not induce Ca2+ release from mitochondria.

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Abbreviations

ATP:

adenosine triphosphate

ADP:

adenosine diphosphate

NADH2 :

β-nicotinamide adenin dinucleotide, reduced form

Mops:

3-(N-morpholino)propane-sulfonic acid

Tris:

tris-(hydroxymethyl)-aminomethane

Hepes:

hydroxyethylpiperazine-N′-2-ethanesulfonic acid

BSA:

bovine serum albumin

EDTA:

(ethylene-dinitrilo)-tetraacetic acid

EGTA:

ethylene glycol-bis(β-aminoethylether)-N,N′-tetraacetic acid

CCCP:

carbonyl cyanide m-chlorophenylhydrazone

DTE:

1,4-dithiothreitol

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  1. Institut für Biologie III der Universität Freiburg, Schänzlestraße 1, D-7800, Freiburg, Federal Republic of Germany

    Peter Dieter & Dieter Marmé

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  1. Peter Dieter
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  2. Dieter Marmé
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Dieter, P., Marmé, D. Ca2+ transport in mitochondrial and microsomal fractions from higher plants. Planta 150, 1–8 (1980). https://doi.org/10.1007/BF00385606

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  • DOI: https://doi.org/10.1007/BF00385606

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