Summary
Crude and alkali-purified fractions of scales isolated from the haptophycean alga Pleurochrysis scherffelii Pringsheim were analyzed. The predominantly cellulosic nature of the fibrillar, alkali-resistent polysaccharide component has been confirmed by gas chromatography of the hydrolysate, by X-ray diffraction and by viscosimetric studies using the nitrified product. Peptide material is covalently linked to this cellulosic structural polysaccharide and remains associated with it through the harsh purification treatments. The amino acid composition of this peptide moiety is given. The relationship of this cellulosic “glycoprotein” to other cell wall glycoproteins is discussed and possible functions during the formation and secretion of wall materials are hypothesized.
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Barnett, J. R., Preston, R. D.: Arrays of granules associated with the plasmalemma in swarmers of Cladophora. Ann. Bot. 34, 1011–1017 (1970).
Bartnicki-Garcia, S.: Cell wall chemistry, morphogenesis, and taxonomy of fungi. Ann. Rev. Microbiol. 22, 87–108 (1968).
Ben-Hayyim, G., Ohad, I.: Synthesis of cellulose by Acetobacter xylinum. VIII. On the formation and orientation of bacterial cellulose fibrils in the presence of acidic polysaccharides. J. Cell Biol. 25, 191–206 (1968).
Brown, R. M.: Observations on the relationship of the Golgi apparatus to wall formation in the marine Chrysophycean alga Pleurochrysis scherffelii Pringsheim. J. Cell Biol. 41, 109–123 (1969).
Brown, R. M., Franke, W. W.: A microtubular crystal associated with the Golgi field of Pleurochrysis scherffelii. (Scale formation in Chrysophycean algae. II.) Planta (Berl.) 96, 354–363 (1971).
Brown, R. M., Franke, W. W., Kleinig, H., Falk, H., Sitte, P.: Cellulosic wall component produced by the Golgi apparatus of Pleurochrysis scherffelii Pringsheim. Science N. Y. 166, 894–896 (1969).
Brown, R. M., Franke, W. W., Kleinig, H., Falk, H., Sitte, P.: Scale formation in Chrysophycean algae. I. Cellulosic and noncellulosic components made by the Golgi apparatus. J. Cell Biol. 45, 246–271 (1970).
Chafe, S. C., Wardrop, A. B.: Microfibril orientation in plant cell walls. Planta (Berl.) 92, 13–24 (1970).
Chrispeels, M. J.: Biosynthesis of cell wall protein: Sequential hydroxylation of proline, glycosylation of hydroxyproline and secretion of the glycoprotein. Biochem. biophys. Res. Commun. 39, 732–737 (1970).
Colvin, J. R.: The biosynthesis of cellulose. In: Zimmermann M. H. ed. The formation of wood in forest trees, p. 189–201. New York: Academic Press 1964.
Franke, W. W.: Seminar given at the Department of Botany and Plant Pathology, Purdue University, Lafayette, Indiana, U.S.A. November 1970.
Franke, W. W., Brown, R. M.: Scale formation in Chrysophycean algae. III. Negatively stained scales of the Coccolithophorid Hymenomonas. Arch. Microbiol. 77, 12–19 (1971).
Franke, W. W., Ermen, B.: Negative staining of plant slime cellulose: An examination of the elementary fibril concept. Z. Naturforsch. 246, 918–922 (1969).
Frei, E., Preston, R. D.: Non-cellulosic structural polysaccharides in algal cell walls. I. Xylan in siphoneous green algae. Proc. roy. Soc. B 160, 293–313 (1964).
Frey-Wyssling, A.: The ultrastructure and biogenesis of native cellulose. L. Zechmeister ed., Progress in the chemistry of organic natural products XXVII, p. 1–30. Wien-New York: Springer 1969.
Green, J. C., Jennings, D. H.: A physical and chemical investigation of the scales produced by the Golgi apparatus within and found on the surface of the cells of Chrysochromulina chiton Parke et Manton. J. exp. Bot. 18, 359–370 (1967).
Günther, I.: Elektronenmikroskopische Untersuchungen an der keimenden Spore von Funaria hygrometrica. J. Ultrastruct. Res. 4, 304–331 (1960).
Heath, M. F., Northcote, D. H.: Glycoprotein of the wall of sycamore tissue-culture cells. Biochem. J. 128, 953–961 (1971).
Husemann, E., Keilich, G.: Charakterisierung der Cellulose aus Quittenkernen. Cellulose Chem. Techn. (Bucharest) 3, 445–468 (1969).
Lamport, D. T.: Cell wall metabolism. Ann. Rev. Plant Physiol. 21, 235–270 (1970).
Lowry, O. H., Rosebrough, N. J., Farr, N. L., Randall, R. L.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Manton, I.: Further observations on scale formation in Chrysochromulina chiton. J. Cell Sci. 2, 411–418 (1967).
Marshall, R. D., Neuberger, A.: Aspects of the structure and metabolism of glycoproteins. Advonc. Carbohyd. Chem. Bioch. 25, 407–478 (1970).
Marx-Figini, M.: Zur Kinetik der Biosynthese der Cellulose in höheren und niederen Pflanzen. Papier 23, 13–14 (1969a).
Marx-Figini, M.: Untersuchungen zur Biosynthese der Cellulose in der Alge Valonia. Biochim. biophys. Acta (Amst.) 177, 27–34 (1969b).
Marx-Figini, M., Schulz, G. V.: Über die Kinetik und den Mechanismus der Biosynthese der Cellulose in den höheren Pflanzen (nach Versuchen an den Samenhaaren der Baumwolle). Biochim. biophys. Acta (Amst.) 112, 81–101 (1966).
Mühlethaler, K.: Ultrastructure and formation of plant cell walls. Ann. Rev. Plant Physiol. 18, 1–24 (1967).
Myers, A., Preston, R. D., Ripley, G. W.: Fine structure in the red algae. I. X-ray and electron microscope investigation of Griffithsia flosculosa. Proc. roy. Soc. B 144, 450–478 (1956).
Northcote, D. H.: The Golgi apparatus, Endeavour 30, No 109, 26–33 (1971a).
Northcote, D. H.: Organization of structure, synthesis and transport within the plant during cell division and growth. Symposia of the society for experimental biology, 25, p. 51–69 Cambridge: At the University Press 1971b.
Northcote, D. H., Pickett-Heaps, J. D.: A function of the Golgi apparatus in polysaccharide synthesis and transport in the root cap cells of wheat. Biochem. J. 98, 159–167 (1966).
Ohad, I., Danon, D.: On the dimensions of cellulose microfibrils. J. Cell Biol. 22, 302–305 (1964).
Percival, E.: Gas chromatography of the trimethylsilyl ethers of some less-common disaccharides. Carboh. Res. 4, 441–443 (1967).
Sentandreu, R., Northcote, D. H.: The structure of a glycopeptide isolated from the yeast cell wall. Biochem. J. 109, 419–432 (1968).
Strauch, L.: Ultramikro-Methode zur Bestimmung des Stickstoffes in biologischem Material. Z. klin. Chem. 5, 165–187 (1965).
Sweely, C. C., Bentley, R., Makita, M., Wells, W. W.: Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. J. Amer. chem. Soc. 85, 2497–2507 (1963).
Wardrop, A. B.: cited in Treiber, E: Die Chemie der Pflanzenzellwand, S. 165, Tab. IV, 6b. Berlin-Göttingen-Heidelberg: Springer 1957.
Weinstock, A., Leblond, C. P.: Elaboration of the matrix glycoprotein of enamel by the secretory ameloblasts of the rat incisor as revealed by radioautography after galactose-3H injection. J. Cell Biol. 51, 26–51 (1971).
Werner, R.: Dissertation am Inst. f. Makrom. Chemie der Universität Freiburg (1964).
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Herth, W., Franke, W.W., Stadler, J. et al. Further characterization of the alkali-stable material from the scales of Pleurochrysis scherffelii: A cellulosic glycoprotein. Planta 105, 79–92 (1972). https://doi.org/10.1007/BF00385167
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DOI: https://doi.org/10.1007/BF00385167