Skip to main content
SpringerLink
Log in

Control of hemicellulose and pectin synthesis during differentiation of vascular tissue in bean (Phaseolus vulgaris) callus and in bean hypocotyl

  • Published:
Planta Aims and scope Submit manuscript

Abstract

Membrane fractions from bean hypocotyl or callus incorporate arabinose from UDP-β-L-arabinose into arabinan and xylose from UDP-α-D-xylose into xylan. The control of these syntheses has been studied during xylogenesis in stele and in xylogenesis induced in callus tissue. Induction of arabinan synthetase activity occurs during division and extension growth while that of xylan synthetase occurs subsequently during the period of secondary thickening of the cell wall. The xylan synthetase induction is correlated with the induction of phenylalanine ammonia-lyase and with lignin synthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PAL:

phenylalanine ammonia-lyase

NAA:

3-naphthylacetic acid

CMD:

medium supplemented with 2,4-dichlorophenoxy-acetic acid and coconut milk

IM:

induction medium

MM:

maintenance medium

EDTA:

ethylendiamine tetracetate

TCA:

trichloroacetic acid

DEAE:

diethylaminoethyl

TLC:

thin layer chromatography

UDP:

uridine diphosphate

References

  • Aspinall, G.O., Cottrell, I.W., Matheson, N.K. (1972) Synthesis of uridine 5-(L-arabino furanosyl pyrophosphate) and the structure of the UDP-L-arabinose formed in plants from UDP-α-D-xylopyranose. Can. J. Biochem. 50, 574–580

    Google Scholar 

  • Bailey, R.W., Hassid, W.Z. (1966) Xylan synthesis from uridine diphosphate-D-xylose by particulate preparations from immature corncobs. Proc. Natl. Acad. Sci. USA, 56, 1586–1593

    Google Scholar 

  • Barrett, A.J., Northcote, D.H. (1965) Apple fruit pectic substances. Biochem. J. 94, 617–627

    Google Scholar 

  • Behrens, N.H., Parodi, A.J., Leloir, L.F. (1971) Glucose transfer from dolichol monophosphate glucose: The product formed with endogenous microsomal acceptor. Proc. Natl. Acad. Sci. USA 68, 2857–2860

    Google Scholar 

  • Bevan, M., Northcote, D.H. (1979a) The interaction of auxin and cytokinin in induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris. Planta 147, 77–81

    Google Scholar 

  • Bevan, M., Northcote, D.H. (1979b) The loss of morphogenic potential and induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris. J. Cell Sci. 39, 339–353

    Google Scholar 

  • Brett, C.T. (1980) The isolation and characterisation of polyprenylphosphatesugars. In: Techniques in carbohydrate biochemistry pp. B305, 1–14 Northcote, D.H., ed. Elsevier, North Holland Biomedical Press, Amsterdam

    Google Scholar 

  • Cleland, W.W. (1967) The statistical analysis of enzyme kinetic data. Adv. Enzymol. 29, 1–32

    PubMed  Google Scholar 

  • Dalessandro, G., Northcote, D.H. (1977a) Changes in enzymic activities of nucleoside diphosphate sugar interconversions during differentiation of cambium to xylem in sycamore and poplar. Biochem. J. 162, 267–279

    PubMed  Google Scholar 

  • Dalessandro, G., Northcote, D.H. (1977b) Changes in enzymic activities of nucleoside diphosphate sugar interconversions during differentiation of cambium to xylem in pine and fir. Biochem. J. 162, 281–288

    PubMed  Google Scholar 

  • Dalessandro, G., Northcote, D.H. (1977c) Possible control of polysaccharide synthesis during cell growth and wall expansion of pea seedlings. Planta 134, 39–44

    Google Scholar 

  • Dalessandro, G., Northcote, D.H. (1981a) Xylan synthetase activity in differentiated xylem cells of sycamore trees (Acer pseudoplatanus). Planta, 151, 53–60

    Google Scholar 

  • Dalessandro, G., Northcote, D.H. (1981b) Increase of xylem synthetase activity during xylem differentiation of the vascular cambium of sycamore and poplar trees. Planta, 151, 61–67

    Google Scholar 

  • Dekker, R.D.H., Richards, G.N. (1976) Hemicelluloses. Their occurrence purification properties and mode of action. In: Advances in carbohydrate chemistry and biochemistry, vol. 32, pp 227–352, Tipson, R.S., Horton, D., eds., Academic Press, New York San Francisco London

    Google Scholar 

  • Dorée, C. (1947) The methods of cellulose chemistry. 2nd. edn., Chapman and Hall, Ltd., London

    Google Scholar 

  • Elbein, A.D. (1969) Biosynthesis of a cell wall glucomannan in mung bean seedlings. J. Biol. Chem. 244, 1608–1616

    PubMed  Google Scholar 

  • Folch, J., Lees, M., Sloane-Stanley, G.H. (1957) A simple method for the isolation and purification of total lipids from animals tissues. J. Biol. Chem. 226, 497–509

    Google Scholar 

  • Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158

    PubMed  Google Scholar 

  • Gorbacheva, I.V., Rodionova, N.A. (1977) Studies on xylan-degrading enzymes. II. Action pattern of endo-1,4-β-xylanase from Aspergillus niger str. 14 on xylan and xylo-oligosaccharides. Biochim. Biophys. Acta 484, 94–102

    Google Scholar 

  • Haddon, L.E., Northcote, D.H. (1975) Quantitative measurement of the course of bean callus differentiation. J. Cell Sci. 17, 11–26

    Google Scholar 

  • Haddon, L.E., Northcote, D.H. (1976a) Correlation of the induction of various enzymes concerned with phenylpropanoid and lignin synthesis during differentiation of bean cells. Planta 128, 255–262

    Google Scholar 

  • Haddon, L.E., Northcote, D.H. (1976b) The effect of growth conditions and origin of tissue on the ploidy and morphogenic potential of tissue cultures of bean (Phaseolus vulgaris L.) J. Exp. Boy. 27, 189–209

    Google Scholar 

  • Hahlbrock, K. (1974) Correlation between nitrate uptake, growth and changes in metabolic activities of cultured plant cells. In: Tissue culture and plant science 1974, pp 363–378. H.E. Street, ed. Academic Press, New York London

    Google Scholar 

  • Harris, P.J., Northcote, D.H. (1970) Patterns of polysaccharide biosynthesis in differentiating cells of maize root-tips. Biochem. J. 120, 479–491

    Google Scholar 

  • Jeffs, R.A., Northcote, D.H. (1966) Experimental induction of vascular tissue in an undifferentiated plant callus. Biochem. J. 101, 146–152

    Google Scholar 

  • Kauss, H. (1967) Biosynthesis of the glucuronic acid unit of hemicellulose B from UDP-glucuronic acid. Biochim. Biophys. Acta 148, 572–574

    Google Scholar 

  • Kuboi, T., Yamada, Y. (1978) Regulation of the enzyme activities related to lignin synthesis in cell aggregates of tobacco cell cultures. Biochim. Biophys. Acta 542, 181–190

    Google Scholar 

  • McNab, J.M., Villemez, C.L., Albersheim, P. (1968) Biosynthesis of galactan by a particulate enzyme preparation from Phaseolus aureus seedlings. Biochem. J. 106, 355–360

    Google Scholar 

  • Neufeld, E.F., Ginsburg, V., Putman, E.W., Fanshier, D., Hassid, W.Z. (1957) Formation and interconversion of sugar nucleotides by plant extracts. Arch. Biochim. Biophys. 69, 602–616

    Google Scholar 

  • Neufeld, E.F., Feingold, D.S., Hassid, W.Z. (1950) Phosphorylation of D-galactose and L-arabinose by extracts from Phaseolus aureus seedlings. J. Biol. Chem. 235, 906–909

    Google Scholar 

  • Northcote, D.H. (1963) Changes in the cell walls during differentiation. Symp. Soc. Exp. Biol. 17, 157–174

    Google Scholar 

  • Northcote, D.H. (1972) Chemistry of the plant cell wall. Annu. Rev. Plant Physiol. 23, 113–132

    Google Scholar 

  • Northcote, D.H. (1979) Biochemical mechanisms involved in plant morphogenesis. In: British plant growth regulator group. Monograph 3, pp 11–20 Control of plant development

  • Northcote, D.H. (1981) Macromolecular aspects of cell wall differentiation. Encyclopaedia of plant physiology. Gene expression in plants. Vol. 16 pp. Springer Verlag, Berlin

    Google Scholar 

  • Odzuk, W., Kauss, H. (1972) Biosynthesis of pure arabinan and xylan. Phytochemistry 11, 2489–2494

    Google Scholar 

  • Ray, P.M. (1980) Cooperative action of β-glucan synthetase and UDP-xylose xylosyl transferase of Golgi membranes in the synthesis of xyloglucan like polysaccharide. Biochim. Biophys. Acta 629, 431–444

    Google Scholar 

  • Rhodes, M.J.C., Wooltorton, L.S.C. (1973) Formation of CoA esters of cinnamic acid derivatives by extracts of Brassica napobrassica root tissue. Phytochemistry 12, 2381–2387

    Google Scholar 

  • Rubery, P.H., Northcote, D.H. (1968) Site of phenylalanine ammonia-lyase activity and synthesis of lignin during xylem formation. Nature (London) 219, 1230–1234

    Google Scholar 

  • Spector, T. (1978) Refinement of the Coomassic Blue method of protein quantitation. Anal. Biochem. 86, 142–146

    PubMed  Google Scholar 

  • Villemez, C.L., Swanson, A.L., Hassid, W.Z. (1966) Properties of a polygalacturonic acid-synthesizing enzyme system from Phaseolus aureus seedlings. Arch. Biochem. Biophys. 116, 446–452

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, CB2 1QW, Cambridge, U.K.

    G. P. Bolwell & D. H. Northcote

Authors
  1. G. P. Bolwell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. H. Northcote
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bolwell, G.P., Northcote, D.H. Control of hemicellulose and pectin synthesis during differentiation of vascular tissue in bean (Phaseolus vulgaris) callus and in bean hypocotyl. Planta 152, 225–233 (1981). https://doi.org/10.1007/BF00385148

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00385148

Key words

Search

Navigation