Abstract
The uptake of [1-3H]geranylgeranyl diphosphate (GGPP) into protoplasts and intact etioplasts and the metabolic interconversion therein was studied after a 2 min pulse of white light. The chlorophyll synthetase reaction, Chlide+GGPP→ChlGG, was taken as a natural probe for the etioplast compartment. This reaction yields labeled ChLGG and, by hydrogenation, labeled ChlP, when [1-3H]GGPP receives access to the etioplast stroma. It was found that penetration across the plastid envelope was rapid and that penetration across the plasma membrane of protoplasts, however, was slow. A cellular pool of soluble GGPP was detected. This pool was lost, in part, during preparation of the protoplasts and almost completely during preparation of the etioplasts. The membrane-bound phytol pool of etioplasts could not be replaced by exogenous [3H]GG. The endogenous GG and phytol pools of protoplasts, which were larger than those of etioplasts, could be replaced in part by exogenous [3H]GGPP. That part of this pool exists as soluble GGPP or as a direct precursor in the cytoplasm is discussed.
Similar content being viewed by others
Abbreviations
- GGPP:
-
geranylgeranyldiphosphate
- ChlGG :
-
geranylgeranyl chlorophyllide a
- ChlP :
-
phytyl chlorophyllide a
- IPP:
-
isopentenyl diphosphate
- Chlide:
-
chlorophyllide a
References
Benz, J. (1980) Untersuchungen zur Chlorophyll-Biosynthese in etiolierten Haferkeimlingen: Veresterung von Chlorophyllid in vitro. PhD thesis, Universität München
Benz, J., Wolf, C., Rüdiger, W. (1980) Chlorophyll biosynthesis: hydrogenation of geranylgeraniol. Plant Sci. Lett. 19, 225–230
Block, M.A., Joyard, J., Douce, R. (1980) Site of synthesis of geranylgeraniol derivatives in intact spinach chloroplasts. Biochim. Biophys. Acta 631, 210–219
Hampp, R. (1981) Rapid separation of the plastid, mitochondrial, and cytoplasmic fractions from intact leaf protoplasts of Avena. Planta (in press)
Hampp, R., Schmidt, H.-W. (1976) Changes in envelope permeability during chloroplast development. Planta 129, 69–73
Hampp, R., Ziegler, H. (1980) On the use of Avena protoplasts to study chloroplast development. Planta 147, 485–494
Rüdiger, W., Hedden, P., Köst, H.-P., Chapman, D.J. (1977) Esterification of chlorophyllide by geranylgeranyl pyrophosphate in a cell-free system from Maize shoots. Biochem. Biophys. Res. Comm. 74, 1268–1272
Rüdiger, W., Benz, J. (1979) Influence of aminotriazol on the biosynthesis of chlorophyll and phytol. Z. Naturforsch. 34c, 1055–1057
Rüdiger, W., Benz, J., Guthoff, C. (1980) Detection and partial characterization of activity of chlorophyll synthetase in etioplast membranes. Eur. J. Biochem. 109, 193–200
Schoch, S., Lempert, U., Rüdiger, W. (1977) Über die letzten Stufen der Chlorophyll-Biosynthese. Zwischenprodukte zwischen Chlorophyllid und phytolhaltigem Chlorophyll. Z. Pflanzenphysiol. 83, 427–436
Schoch, S. (1978) The esterification of chlorophyllide a in greening bean leaves. Z. Naturforsch. 33c, 712–714
Schoch, S., Hehlein, C., Rüdiger, W. (1980) Influence of anaerobiosis on chlorophyll biosynthesis in greening oat seedlings (Avena sativa L.) Plant Physiol. 66, 576–579
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Benz, J., Hampp, R. & Rüdiger, W. Chlorophyll biosynthesis by mesophyll protoplasts and plastids from etiolated oat (Avena sativa L.) leaves. Planta 152, 54–58 (1981). https://doi.org/10.1007/BF00384985
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00384985