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Carbon isotope composition of biochemical fractions isolated from leaves of Bryophyllum daigremontianum berger, a plant with crassulacean acid metabolism: Some physiological aspects related to CO2 dark fixation

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Abstract

Isotope analysis of the biochemical fractions isolated quantitatively from young and mature leaves of Bryophyllum daigremontianum Berger have been carried out before and after a dark period of accumulation of organic acids. The mature leaf is enriched in 13C compared to the young leaf. The δ13C values of the different leaf constituents vary between the δ13C values of C4 plants (-11‰) and those of C3 plants (-27‰). During the dark period, the two types of leaves store organic acids with δ13C values of ≃-15‰ and lose insoluble sugars, including starch with a δ13C value of ≃-12‰. Furthermore, young leaves store phosphorylated compounds with δ13C values of ≃-11‰ and lose weakly polymerised sugars with δ13C values of ≃-18‰. These results led to the formulation of a hypothesis of the origin of the two substrates of β-carboxylation: phosphoenolpyruvate arises from the glycolytic breakdown of the insoluble sugars rich in 13C, and the major portion of the CO2 is the result of the complete breakdown (respiration) of the soluble sugars rich in 12C. The existence of two independent sugar pools leads to the assumption that there are two separate glycolytic pathways. The 13C enrichment of the stored products of the young leaves in the day seems to be the result of a weak discrimination for 13C by ribulose diphosphate carboxylase, which reassimilates to a great extent the CO2 released from malate accumulated in the night.

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Abbreviations

CAM:

crassulacean acid metabolism

C3 metabolism:

metabolism with primary carbon fixed by the Calvin and Benson pathway

C4 metabolism:

metabolism with primary carbon fixed by the Hatch and Slack pathway

δ13C(‰):

(Rsample-RPDB) 103/RPDB where PDB=Pee Dee belemnite (belemite from the Pee Dee formation South Carolina) and R=13C/12C

NAD-MDH(EC1.1.1.37):

NAD-malate dehydrogenase

NADP-ME (EC1.1.1.40):

NADP-malic enzyme

PEP:

phosphoenolpyruvate

PEPC (EC4.1.1.31):

PEP carboxylase

PGA:

phosphoglyceric acid

Py.di-PK(EC2.7.9.1):

pyruvate, Pi-dikinase

RuDP:

ribulose diphosphate

RuDPC (EC4.1.1.39):

RuDP carboxylase

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  1. Laboratoire de Physiologie Cellulaire Végétale, associé au C.N.R.S., dépendant de l'Université de Paris XI, Center d'Orsay, Bât, 430, F-91405, Orsay Cedex, France

    Eliane Deleens & Jeannine Garnier-Dardart

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  1. Eliane Deleens
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  2. Jeannine Garnier-Dardart
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Deleens, E., Garnier-Dardart, J. Carbon isotope composition of biochemical fractions isolated from leaves of Bryophyllum daigremontianum berger, a plant with crassulacean acid metabolism: Some physiological aspects related to CO2 dark fixation. Planta 135, 241–248 (1977). https://doi.org/10.1007/BF00384896

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