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Rapid differentiation of tracheary elements in cultured explants of Jerusalem artichoke

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Abstract

the culture of Jerusalem artichoke (Helianthus tuberosus L.) tuber explants on filter paper discs moistened with liquid medium resulted in rapid and consistent xylem differentiation. The number of tracheary elements increased in discrete steps, the first at 48 h with a second at 56–58 h, following partially synchronous mitoses at 20 and 30 h. Factors favouring xylem cell differentiation were optimum levels of both an auxin and a cytokinin, low medium nitrogen concentrations, small volumes of medium, and high culture temperatures. A cell counting method employing Feulgen-stained nuclei and suitable for quantifyings small numbers of immature tracheary elements is described.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

α-naphthalene acetic acid

BAP:

benzylaminopurine

GA3 :

gibberellic acid

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Authors and Affiliations

  1. Department of Botany and Microbiology, University College London, Gower Street, WCIE 6BT, London, UK

    R. Phillips & J. H. Dodds

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  1. R. Phillips
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  2. J. H. Dodds
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Phillips, R., Dodds, J.H. Rapid differentiation of tracheary elements in cultured explants of Jerusalem artichoke. Planta 135, 207–212 (1977). https://doi.org/10.1007/BF00384891

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  • DOI: https://doi.org/10.1007/BF00384891

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