Summary
Antimycin-resistant (AR) mutants of the yeast Kluyveromyces lactis, obtained either spontaneously or after manganese treatment, were isolated and genetically characterized. Most of the mutants obtained after manganese mutagenesis and two spontaneous mutants, tolerated high antimycin concentrations (more than 10 /gmg/ml) and were extrachromosomal. One mutant which grew only in low antimycin (1 /gmg/ml) showed a Mendelian type of inheritance. The extrachromosomal mutants could be assigned to at least two genetic loci (A RI and A RII ). Mutants representative of these two groups showed increased resistance to the antibiotic when the respiration of whole cells or mitochondria was studied. Extrachromosomal mutants of Saccharomyces cerevisiae resistant to antimycin were also induced with manganese, isolated and characterized. Comparative studies of the antimycin-resistant mutants of K. lactis and S. cerevisiae permitted the following observations: a) K. lactis is more resistant to antimycin, funiculosin, mucidin and diuron than S. cerevisiae, as are the AR mutants; b) K. lactis shows correlated sensitivity to funiculosin differing in this aspect from S. cerevisiae; c) the antimycin-resistant mutants of K. lactis belonging to group 11 (A RII ) were also resistant to diuron, tolerating concentrations of more than 200 /gmg/ml; d) all extrachromosomal antimycin-resistant-mutants of S. cerevisiae and some of the AR mutants of K. lactis were more sensitive to mucidin than the wild type.
Similar content being viewed by others
Abbreviations
- diuron or DCMU:
-
3-(3,4-Dichlorophenyl)-1,1dimethylurea
- HQNO:
-
2-n Heptyl-4-hydroxyquinoline N-oxide
References
Berden JA, Slater EC (1972) Biochim Biophys Acta 256:199–215
Brunner A, Tuena de Cobos A, Griffiths DE (1977) Mol Gen Genet 152:183–191
Brunner A, Tuena de Cobos A (1980) Mol Gen Genet 178:351–355
Bulder CJEA (1964) J Microbiol Serol 30:1–9
Burger G, Lang B, Bandlow W, Schweyen RJ, Backhaus B, Kaudewitz F (1976) Biochem Biophys Res Commun 72: 1201–1208
Burger G, Wolf K (1981) Mol Gen Genet 181:134–139
Butow RA, Zeydel M (1968) J Biol Chem 243:2545–2549
Celis E, Mas J, Brunner A (1975) Genet Res Cambridge 25:59–69
Chevillotte-Brivet P, Meunier-Lemesle D, Forget N, Pajot P (1983) Eur J Biochem 129:653–661
Colson AM, Luu The Van, Convent B, Briquet M, Goffeau A (1977) Eur J Biochem 74:521–526
Colson AM, Slonimski PP (1979) Mol Gen Genet 167:287–298
Convent B, Briquet M (1978) Eur J Biochem 82:473–481
Corbett JR, Wright K, Baillie AC (1984) The biochemical mode of action of pesticides, 2nd ed. Academic, London, p 50
Deutsch J, Dujon B, Netter P, Petrochilo E, Slonimski PP, Bolotin-Fukuhara M, Coen D (1974) Genetics 76:195–219
Dujon B (1981) Mitochondrial Genetics and Functions. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces. Life Cycle and Inheritance 1, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 575–584
Ferrero I, Viola AM, Goffeau A (1981) J Microbiol Serol 47: 11–24
Gillham N (1978) Organelle heredity. Raven, New York
Grimmelikhuijzen CJP, Slater EC (1973) Biochim Biophys Acta 305:67–79
Gutweniger H, Bisson R, Montecucco C (1981) J Biol Chem 256:11132–11136
Heritage J, Whittaker PA (1977) Mol Gen Genet 156:93–98
Heslot H, Goffeau A, Louis C (1970) J Bacteriol 104:473–481
Lang B, Burger G, Wolf K, Bandlow W, Kaudewitz F (1975) Mol Gen Genet 137:353–363
Linnane AW, Sanders G, Gingold EB, Lukins HB (1968) Proc Natl Acad Sci USA 59:903–910
Mattoon JR, Balcavage WX (1967) Methods Enzymol 10:135–142
Michaelis G (1976) Mol Gen Genet 146:133–137
Morgan AJ, Whittaker PA (1978) Mol Gen Genet 164:185–193
Moser UK, Walter P (1975) FEBS Letters 50:279–282
Pratje E, Michaelis G (1977) Mol Gen Genet 152:167–174
Putrament A, Baranowska H, Ejchart A, Prazmo W (1975) J Gen Microbiol 62:265–270
Rank GH, Bech-Hansen NT (1973) Mol Gen Genet 126:93–102
Rieske JS (1976) Biochim Biophys Acta 456:195–247
Roberts H, Smith SC, Marzuki S, Linnane AW (1980) Arch Biochem Biophys 200:387–395
Sager R (1972) Cytoplasmic genes and organelles. Academic New York
Sherman F, Fink GR, Lawrence CW (1974) Methods in yeast genetics. Cold Spring Harbor Laboratories, Cold Spring Harbor, NY
Sriprakash KS, Batum C (1981) Curr Genet 4:73–80
Šubík J, Behún M, Musilek V (1974) Biochem Biophys Res Commun 57:17–22
Šubík J, Tákacsová G (1978) Mol Gen Genet 161:99–108
Tákacsová G, Šubík J, Zbigniew K (1980) Mol Gen Genet 179: 141–146
Thierbach G, Reichenbach H (1981) Biochim Biophys Acta 638:282–289
Thierbach G, Michaelis G (1982) Mol Gen Genet 186:501–506
Tzagoloff A (1982) Mitochondria. Plenum, New York
Wainio WW (1970) The mammalian mitochondrial respiratory chain. Academic, New York, pp 235
Wallace DC (1982) Microbiol Rev 46:208–240
Wolf K, Burger G, Lang B, Kaudewitz F (1976) Mol Gen Genet 144:67–73
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brummer, A.L., Mendoza, V.R. & Tuena de Cobo, A. Extrachromosomal genetics in the yeast Kluyveromyces lactis. Isolation and characterization of antimycin-resistant mutants. Curr Genet 11, 475–482 (1987). https://doi.org/10.1007/BF00384609
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00384609