Abstract
The dependence of phenylalanine ammonialyase (PAL) induction in bean suspension cultures on the concentration of naphthylacetic acid (NAA) and kinetin has been investigated and the timing of the effect of each hormone has been determined. NAA was required at an optimal concentration of 1 mg l-1 2 days prior to the increase in PAL activity. Kinetin caused a prapid stimulation of the rate of PAL induction and the total amount of PAL induced in a concentration range of 0.1–0.5 mg l-1 when it was supplied to the cells immediately prior to the expected rise in PAL activity. The inhibitory effect of 2 mg l-1 NAA on PAL induction was overcome by an increased concentration of kinetin.
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Abbreviations
- NAA:
-
naphthylacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3yl acetic acid
- PAL:
-
phenylalanine ammonia-lyase (EC 4.3.1.5.)
References
Dixon, R.A., Fuller, K.W.: Effects of synthetic auxin levels on phaseollin production and PAL activity in tissue cultures of Phaseolus vulgaris L. Physiol. Plant Pathol. 9, 299–312 (1976)
Fosket, D.E., Torrey, J.G.: Hormonal control of cell proliferation and xylem differentiation in cultured tissues of Glycine max. var. Biloxi. Plant Physiol. 44, 871–890 (1969)
Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50, 151–158 (1968).
Haddon, L., Northcote, D.H.: Quantitative measurement of the course of bean callus differentiation. J. Cell Sci. 17, 11–26 (1975)
Haddon, L., Northcote, D.H.: Correlation of the induction of various enzymes concerned with phenylpropanoid and lignin synthesis during differentiation of bean cells. Planta 128, 255–262 (1976)
Kubei, T., Yamada, Y.: Regulation of the enzyme activities related to lignin synthesis in cell aggregates of tobacco cell cultures. Biochim. Biophys. Acta 542, 181–190 (1978)
Rhodes, M.J.C., Wooltorton, L.S.C.: Formation of CoA esters of cinnamic acid derivatives by extracts of Brassica napo-brassica root tissue. Phytochemistry 12, 2381–2387 (1973)
Rubery, P.H., Northcote, D.H.: Site of phenylalanine ammonialyase activity and synthesis of lignin during xylem formation. Nature 219, 1230–1234 (1968)
Schröder, J.: Light-induced increase of messenger RNA for plenylalanine ammonia-lyase in cell suspension cultures of Petroselinum hortense. Arch. Biochem. Biophys. 182, 488–496 (1977)
Schröder, J., Betz, B., Hahlbrock, K.: Light-induced enzyme synthesis in suspension cultures of Petroselinum hortense. Eur. J. Biochem. 67, 527–541 (1976)
Schröder, J., Betz, B., Hahlbrock, K.: Messenger RNA controlled increase of phenylalanine ammonia-lyase activity in parsley. Plant Physiol. 60, 440–445 (1977)
Skoog, F., Miller, C.O.: Chemical regulation of growth and organ formation in plant tissue cultures in vitro. Symp. Soc. Exp. Biol. 11, 118–181 (1957)
Spector, T.: Refinement of the Coomassie Blue method of protein estimation. Anal. Biochem. 86, 142–146 (1978)
Wright, K., Northcote, D.H.: Induced root formation in sycamore callus. J. Cell Sci. 11, 319–337 (1972)
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Bevan, M., Northcote, D.H. The interaction of auxin and cytokinin in the induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris . Planta 147, 77–81 (1979). https://doi.org/10.1007/BF00384594
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DOI: https://doi.org/10.1007/BF00384594