Abstract
The relative amounts of Concanavalin A (Con A) bound by gamete and vegetative flagella of both mating types (mt + and mt -) of Chlamydomonas eugametos were determined using 125I-Con A. Con A agglutinated all cell types by cross-linking their flagella in a random manner. No correlation was found between the extent of Con A-binding and Con A-mediated isoagglutination. Con A inhibited the sexual interaction between gametes at various levels. In mt + gametes it blocked sexual agglutination, whereas in mt - gametes it prevented papillar fusion. By SDS-gel electrophoresis nine Con A-binding components were found to be present in flagella. However, it was not possible to allocate a role in sexual agglutination to any of these components since they were present in all cell types, including vegetative cells which are not able to sexually agglutinate.
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Abbreviations
- Con A:
-
concanavalin A
- SDS:
-
sodium dodecyl sulphate
- TB:
-
Tris buffer
- PBS:
-
phosphate buffered saline
- HRP:
-
horse radish peroxidase
- SEM:
-
scanning electron microscope
- PAS:
-
periodic acid Schiff
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Musgrave, A., van der Steuyt, P. & Ero, L. Concanavalin A binding to Chlamydomonas eugametos flagellar proteins and its effect on sexual reproduction. Planta 147, 51–56 (1979). https://doi.org/10.1007/BF00384590
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DOI: https://doi.org/10.1007/BF00384590