Summary
A total of 27 out of 28 BamHI restriction fragments of N. otophora ct-DNA have been stably cloned into plasmid pBR322. Each cloned fragment was identified by its electrophoretic mobility. By using this cloned library and a second restriction enzyme, SmaI, a physical map of N. otophora ct-DNA, was also constructed. The genes for rRNA and the LS of RuBPCase have been localized in the clone and marked on the map. No unusual structural feature of Nicotiana ct-DNA was detected in this species. Attempts to perform the in vivo expression of the cloned fragments employing the plasmid-directed protein synthesis system in maxicells met with some success. It is clearly demonstrated that certain cloned ct-DNA fragments could direct the synthesis of chloroplast polypeptides in E. coli.
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Zhu, Y.S., Duvall, E.J., Lovett, P.S. et al. Nicotiana chloroplast genome. Molec. Gen. Genet. 187, 61–66 (1982). https://doi.org/10.1007/BF00384384
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DOI: https://doi.org/10.1007/BF00384384