Summary
The cloned recA + gene of Proteus mirabilis substitutes for a defective RecA protein in Escherichia coli recA − mutants, and restores recombination, repair and phage induction functions to near normal levels. In a previous report, we described the purification and charactrisation of the recombination activities of the P. mirabilis RecA protein (West et al. 1983b). In this paper, we show that the purified protein catalyses the cleavage of both the Escherichia coli LexA protein and the bacteriophage lambda repressor in vitro. These results provide a direct biochemical basis for the interspecies complementation observed in vivo and suggest that P. mirabilis has an SOS regulatory network similar to that of E. coli.
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Cox MM, McEntee KM, Lehman IR (1981) A simple and rapid method for the large scale purification of the RecA protein of Escherichia coli. J Biol Chem 256:4676–4678
Craig NL, Roberts JW (1980) E. coli RecA protein-directed cleavage of phage λ repressor requires polynucleotide. Nature 283:26–30
de Vos WM, Venema G (1983) Transformation of Bacillus subtilis competent cells: Identification and regulation of the recE gene product. Mol Gen Genet 190:56–64
Eitner G, Solonin AS, Tanyashin VI (1981) Cloning of a recA-like gene of Proteus mirabilis. Gene 14:301–308
Eitner G, Adler B, Lanzov VA, Hofemeister J (1982) Interspecies recA protein substitution in Escherichia coli and Proteus mirabilis. Mol Gen Genet 185:481–486
Horii T, Ogawa T, Nakatani T, Hase T, Matsubara H, Ogawa H (1981) Regulation of SOS functions: Purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein. Cell 27:515–522
Little JW (1983) The SOS regulatory system: Control of its state by the level of RecA protease. J Mol Biol 167:791–808
Little JW, Mount DW (1982) The SOS regulatory system of Escherichia coli. Cell 29:11–22
Little JW, Edmiston SH, Pacelli LZ, Mount DW (1980) Cleavage of Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci USA 77:3225–3229
Little JW, Mount DW, Yanisch-Perron CR (1981) Purified lexA protein is a repressor of the recA and lexA genes. Proc Natl Acad Sci USA 78:4199–4203
Pierre A, Paoletti C (1983) Purification and characterization of recA protein from Salmonella typhimurium. J Biol Chem 258:2870–2874
Radding CM (1982) Homologous pairing and strand exchanges in genetic recombination. Ann Rev Biochem 16:405–437
Roberts JW, Roberts CW, Craig NL (1978) Escherichia coli recA gene product inactivates phage λ repressor. Proc Natl Acad Sci USA 75:4714–4718
Sancar A, Stachelak C, Konigsberg W, Rupp WD (1980) Sequences of the recA gene and protein. Proc Natl Acad Sci USA 77:2611–2615
West SC, Countryman JK, Howard-Flanders P (1983a) Enzymatic formation of biparental figure-eight molecules from plasmid DNA and their resolution in E. coli. Cell 32:817–829
West SC, Countryman JK, Howard-Flanders P (1983b) Purification and properties of the RecA protein of Proteus mirabilis. J Biol Chem 258:4648–4654
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Communicated by P.T. Emmerson
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West, S.C., Little, J.W. P. mirabilis RecA protein catalyses cleavage of E. coli LexA protein and the λ repressor in vitro. Molec. Gen. Genet. 194, 111–113 (1984). https://doi.org/10.1007/BF00383505
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DOI: https://doi.org/10.1007/BF00383505