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Ribosomal RNA synthesis in uninfected and SPO1am34 infected Bacillus subtilis

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Summary

The percent of transcription which is ribosomal RNA in Bacillus subtilis has been measured by hybridization of pulse labeled RNA to a specific DNA probe carrying the 3′ end of the 23S RNA gene. Ribosomal RNA synthesis increased with cellular growth rate, and showed a rapid increase after a nutritional shift up.

RNA synthesis in SPO1am34 infected cells was also examined. Infected cells continue to synthesize ribosomal RNA at the preinfection rate, but cannot respond to media enrichment by increasing the percent ribosomal RNA synthesis.

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  1. Department of Microbiology, University of British Columbia, V6T 1W5, Vancouver, British Columbia, Canada

    Vera B. Webb & George B. Spiegelman

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  1. Vera B. Webb
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  2. George B. Spiegelman
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Communicated by A. Böck

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Webb, V.B., Spiegelman, G.B. Ribosomal RNA synthesis in uninfected and SPO1am34 infected Bacillus subtilis . Molec. Gen. Genet. 194, 98–104 (1984). https://doi.org/10.1007/BF00383503

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  • DOI: https://doi.org/10.1007/BF00383503

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