Abstract
When Bacillus subtilis is infected by bacteriophage SPO1, the phage directs the remodeling of the host cell, converting it into a factory for phage reproduction. Much synthesis of host DNA, RNA, and protein is shut off, and cell division is prevented. Here I describe the protocols by which we have demonstrated those processes, and identified the roles played by specific SPO1 gene products in causing those processes.
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Stewart, C.R. (2018). Analysis of Host-Takeover During SPO1 Infection of Bacillus subtilis . In: Clokie, M., Kropinski, A., Lavigne, R. (eds) Bacteriophages. Methods in Molecular Biology, vol 1681. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7343-9_2
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DOI: https://doi.org/10.1007/978-1-4939-7343-9_2
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Online ISBN: 978-1-4939-7343-9
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