Summary
One hundred sera of individuals infected with Schistosoma mansoni and/or S. haematobium were examined for the presence of specific anti Schistosoma antibodies by means of different immunoprecipitation techniques: immunoelectrophoresis, immunodiffusion, immunoelectroosmophoresis (on two different supports), and electroimmunodiffusion. The immunoelectroosmophoresis proved to be superior to the other immunoprecipitation techniques, its main advantages being sensitivity, rapidity, and economic use of reagents. Precipitins against the antigen of the intermediate host, Biomphalaria glabrata, were demonstrated in 66% of the sera.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ambroise-Thomas, P.: Etude séro-immunologique de dix parasitoses par les techniques d'immuno-fluorescence. Thesis, Lyon 1969
Browne, H. G., Thomas, J. I.: A method for isolating pure, viable Schistosoma eggs from host tissues. J. Parasit. 49, 371–374 (1963)
Capron, A., Biguet, J., Vernes, A., Afchain, D.: Structure antigénique des helminthes. Aspects immunologiques des rélations hôte-parasite. Path. et Biol. 16, 121–138 (1968)
Capron, A., Vernes, A., Biguet, J., Rose, F., Clay, A., Adenis, L.: Les précipitines seriques dans les bilharzioses humaines et expérimentales à Schistosoma mansoni, S. haematobium et S. japonicum. Ann. Parasit. hum. comp. 41, 123–187 (1966)
Deelder, A. M.: Immunology of experimental infections with Schistosoma mansoni in the Swiss mouse and with Fasciola hepatica in the rabbit. Acta leidensia 39, 1–107 (1973)
Deelder, A. M., Ploem, J. S.: An immunofluorescence reaction for Schistosoma mansoni using the Defined Antigen Substrate Spheres (DASS) system. J. immunol. Meth. 4, 239–251 (1974)
Deelder, A. M., Snoijink, J. J., Ploem, J. S.: Immunoprecipitation and class-specific immunofluorescence titration of human serum antibodies to Schistosoma mansoni antigens. Z. Parasitenk. 46, 195–201 (1975)
Deelder, A. M., Streefkerk, J. G.: Schistosoma mansoni: Immunohistoperoxidase procedure in defined antigen substrate spheres (DASS) system as serologic field test. Exp. Parasit. 37, 405–410 (1975)
Despommier, D., Müller, M., Jenks, B., Fruitstone, M.: Immunodiagnosis of human trichinosis using counterelectrophoresis and agar gel diffusion techniques. Amer. J. trop. Med. Hyg. 23, 41–44 (1974)
Gentilini, M., Pinon, J.-M., Niel, G.: Immuno-électrodiffusion sur membrane d'acétate de cellulose: applications en parasitologie. Bull. Soc. Path. exot. 65, 60–66 (1972)
Hillyer, G. V.: Immunoprecipitins in Schistosoma mansoni infections.. IV. Human infections. Exp. Parasit. 25, 376–381 (1969)
Krupp, I. M.: Comparison of counterimmunoelectrophoresis with other serologic tests in the diagnosis of amebiasis. Amer. J. trop. Med. Hyg. 23, 27–30 (1974)
Levine, D. L.: Studies on the immunology and serology of schistosomiasis. Diss. Abstr. 19, 2694 (1959)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
McCullough, F. S., Bradley, D. J.: Egg output stability and the epidemiology of Schistosoma haematobium. Part I. Variation and stability in Schistosoma haematobium egg counts. Trans. roy. Soc. trop. Med. Hyg. 67, 475–490 (1973)
Scott, T. A., Melvin, E. H.: Determination of dextran with anthrone. Analyt. Chem. 25, 1656–1661 (1953)
Sepúlveda, B., Aubanel, M., Landa, L., Velázquez, G.: Avances en la técnica de contra-immuno-electroforesis para el estudio serológico de la amibiasis. Arch. Invest. Méd. 3, (suppl.) 363–370 (1972)
Smithers, S. R.: Gel-diffusion studies on Schistosoma mansoni. Trans. roy. Soc. trop. Med. Hyg. 54, 8 (1960)
Svendsen, P. J., Axelsen, N. H.: A modified antigen-antibody crossed electrophoresis characterizing the specificity and titre of human precipitins against Candida albicans. J. immunol. Meth. 1, 169–176 (1972)
Sweet, G., Wilson, D. E., Gerber, J. D.: Application of electroimmunodiffusion and crossed electroimmunodiffusion to the comparative serology of a microorganism (Histoplasma capsulatum). J. Immunol. 111, 554–564 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Deelder, A.M., Kornelis, D., Rier, W.B. et al. Immunodiagnosis of human schistosomiasis using different immunoprecipitation techniques. Z. F. Parasitenkunde 47, 111–118 (1975). https://doi.org/10.1007/BF00382634
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00382634