Abstract
Schistosomiasis is a neglected tropical parasitic disease which has been controlled by praziquantel for many decades; however, reemergence of praziquantel resistant strains has been a continuous threat. Therefore, the development of reliable antischistosomal vaccine is significantly demanded for optimal control. In the present study, comparison among Schistosoma haematobium, Schsitosoma mansoni and Pygidiopsis genata crude antigens was carried out by Sodium dodecyl sulphate polyacrylamide gel electrophoresis. Hyperimmunization of rabbits with tested parasites’ crude antigens was done to obtain hyperimmune sera. Western blotting was applied to show cross reactivity between parasites’ crude antigens and either homologous or heterologous sera. Although there was no cross reaction between P. genata crude antigens and sera of both Schistosoma species and vice versa; it is supposed that the immunogenic band at 79 kDa might develop cross reactivity with Schistosma spp. SEA fractionation if used in future studies.
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Acknowledgements
Authors would like to express their deepest gratitude and thanks to Prof Ahmed Kamal Dyab, Head of Medical Parasitology Department, Assiut University, Egypt for his assistance in sing all the department’s equipment and reagents and Prof Mervat Al-Azzoni, (former head of Medical Parasitology Department) Faculty of Medicine Alexandria University, Egypt for her assistance in getting the needed infected fishes and supplying us with her fantastic Ph.D. thesis.
Funding
This study was financed by the Grant office of Faculty of Medicine, Assiut University, Egypt (Reference code: 1168). The authors, therefore, acknowledge with thanks the Grant Office for technical and financial support.
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AHAH: Collection of materials, conducting all practical experiments and manuscript writing. TMH: Supervision of the experimental studies. LAG: Planning the study design and leading the research team. RMAK: Proposal of the study protocol and final manuscript revision.
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The study protocol and all steps of practical experiments were approved by the Research and Ethics Committee of Faculty of Medicine, Assuit University.
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Hegazy, A.H.A., Galal, L.A., Hassan, T.M. et al. Reliability of heterophyid antigens in heterologous protection against human schistosomiasis. J Parasit Dis 44, 349–354 (2020). https://doi.org/10.1007/s12639-020-01195-8
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DOI: https://doi.org/10.1007/s12639-020-01195-8