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The structure and cytochemistry of the neurosecretory cells of Fasciola gigantica cobbold and Fasciola hepatica L.

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Summary

Histochemical studies of the nervous system of Fasciola gigantica and Fasciola hepatica were undertaken. Neurosecretory cells were detected by Gomori's aldehydefuchsin, Bargmann's chrome hematoxylin-phloxin, Mallory's triple stain, periodic acid-Schiff, Heidenhain's Azan and alcian blue after potassium permanganate oxidation.

Two types of neurosecretory cells were recognized and designated as “A” and “B”. Type “A” cells occurred in small numbers in the brain and subesophageal mass and type “B” cells were ubiquitous in distribution. The reactions of these cells to the standard stains for neurosecretory substance generally, were less intense than the neurosecretory cells of other animals such as crustaceans and insects. The structure, organisation, distribution and cytochemistry of neurosecretory cells in Fasciola gigantica and Fasciola hepatica is discussed.

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References

  • Adams, C. M. M., Sloper, J. C.: The hypothalamic elaboration of posterior pituitary principles of man, rat and dog. Histochemical evidence derived from a performic acid alcian blue reaction for cystine. J. Endocr. 13, 221–228 (1956)

    Google Scholar 

  • Baker, J. R.: The histochemical recognition of lipine. Quart. J. micr. Sci. 87, 441–469 (1946)

    Google Scholar 

  • Bargmann, W.: Über die neurosekretorische Verknüpfung von Hypothalamus und Neurohypophyse. Z. Zellforsch. 34, 610–634 (1949)

    PubMed  Google Scholar 

  • Bern, H. A., Hagadorn, I. R.: Neurosecretion, vol. 1. In: T. H. Bullock and G. A. Horridge, eds., p. 353–429. San Francisco and London: W. H. Freeman & Co. 1965

    Google Scholar 

  • Brachet, J.: The use of basic dyes for the cytochemical detection of RNA. Quart. J. micr. Sci. 94, 1–10 (1953)

    Google Scholar 

  • Bullock, T. H., Horridge, G. A.: Structure and function in the nervous system of invertebrates, vol. 1, p. 353–429. San Francisco and London: W. H. Freeman & Co. 1965

    Google Scholar 

  • Davey, K. G.: Neurosecretory cells in a nematode, Ascaris lumbricoides. Canad. J. Zool. 42, 731–734 (1964)

    Google Scholar 

  • Davey, K. G.: Neurosecretion and moulting in some parasitic nematodes. Amer. Zoologist 6, 243–249 (1966)

    Google Scholar 

  • Davey, K. G., Brechenridge, W. R.: Neurosecretory cells in a cestode Hymenolepis diminuta. Science 158, 931–932 (1967)

    PubMed  Google Scholar 

  • Gabe, M.: Neurosecretion, In: G. A. Kerkut, ed., p. 1–872. Oxford-London-New York: Pergamon Press 1966

    Google Scholar 

  • Gomori, G.: Aldehyde-fuchsin a new stain for elastic tissue. Amer. J. clin. Path. 20, 665–666 (1950)

    Google Scholar 

  • Grasso, M.: Prime indagini sulla presenza di cellule neurosecretrici in Fasciola hepatica. R. C. Accad. Naz. Lincei 42, 85–87 (1967a)

    Google Scholar 

  • Grasso, M.: Prime indagini sulla presenza di cellule neurosecretrici in Fasciola hepatica. R. C. Accad. Naz. Lincei 42, 903–905 (1967b)

    Google Scholar 

  • Grasso, M.: Ultrastructural studies in neurosecretion and gamete ripening in platyhelminthes. Gen. comp. Endocr. 18, 3 (1972)

    Google Scholar 

  • Gresson, R., Threadgold, L.: The large neurons and interstitial material of Fasciola hepatica L. Proc. roy. Soc. Edinb. 42, 261–266 (1964)

    Google Scholar 

  • Gurr, G.T.: Staining animal tissues, practical and theoretical. London: Leonard Hill (Books) 1962

    Google Scholar 

  • Herlant-Meewis: Morphologie des systèmes neurosécréteurs chez les invertébrés. Proc. 2nd Intern. Congr. Endocrinol. 83, 556–562 (1964)

    Google Scholar 

  • Hotchkiss, R. D.: A microchemical reaction resulting in the staining of polysaccharide structures in fixed tissue preparations. Arch. Biochem. 16, 131–141 (1948)

    Google Scholar 

  • Lender, T. H., Klein, N.: Mise en évidence de cellules sécrétrices dans le cerveau de la planaire Polycelis nigra. C. R. Acad. Sci. (Paris) 253, 331–333 (1961)

    Google Scholar 

  • Matskasi, I.: On the neurosecretory cells of Opisthodiscus diplodiscoides Cohn (Trematodes) and their structural changes during the day. Folia parasit. 17, 25–30 (1970)

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry, theoretical and applied, vol. I. London: J. & A. Churchill 1968

    Google Scholar 

  • Rogers, W. P.: Neurosecretory cells in the infective stages of Haemonchus contortus. Parasitology 58, 657–662 (1968)

    Google Scholar 

  • Shyamasundari, K.: A method for the simultaneous distinction of neurosecretory and mucoid substances in invertebrates. Curr. Sci. 41, 78 (1972)

    Google Scholar 

  • Steedman, H. F.: Alcian blue, a new stain for mucin. Quart. J. micr. Sci. 91, 477–479 (1950)

    Google Scholar 

  • Stunkard, H. W.: Induced gametogenesis in a monogenetic trematode Polystoma stella Vigueras 1955. J. Parasit. 45, 389–394 (1959)

    Google Scholar 

  • Ude, J.: Neurosekretorische Zellen im Cerebralganglion von Dicrocoelium lanceatum St. u. H. (Trematoda, Digenea). Zool. Anz. 169, 455–457 (1962)

    Google Scholar 

  • Ude, J.: Untersuchungen zur Neurosekretion bei Dendrocoelum lacteum Oerst (Platyhelminthe, Turbellaria). Z. Wiss. Zool. 170, 223–255 (1964)

    Google Scholar 

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Shyamasundari, K., Hanumantha Rao, K. The structure and cytochemistry of the neurosecretory cells of Fasciola gigantica cobbold and Fasciola hepatica L.. Z. F. Parasitenkunde 47, 103–109 (1975). https://doi.org/10.1007/BF00382633

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