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Immunogold labelling of the cytoplasmic estradiol receptor in resting porcine endometrium

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Summary

Serial sections of resting porcine endometrium were analyzed with the monoclonal antibody 13H2 using goat antimouse IgG/5 nm gold as secondary reagent or with either polyclonal antibodies from goat #402 or the rat monoclonal antibody H222, both in combination with protein G/12 nm gold. A modestly higher labelling of nuclei than of cytoplasm was seen only with the monoclonal antibody H222. Polyclonal #402 and monoclonal 13H2 showed fewer attachments over nuclear than over cytoplasmic areas. The highest densities of attachment and of predominantly cytoplasmic labelling were obtained with the monoclonal antibody 13H2. The results confirm the earlier assumption of a restricted accessiblity of estradiol receptor in the cytoplasm of resting cells for immunoreagents.

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Authors and Affiliations

  1. Max-Planck-Institut für Experimentelle Endokrinologie, P.O. Box 610309, W-3000, Hannover 61, Federal Republic of Germany

    Walter D. Sierralta & Hubert H. Thole

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  1. Walter D. Sierralta
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  2. Hubert H. Thole
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Sierralta, W.D., Thole, H.H. Immunogold labelling of the cytoplasmic estradiol receptor in resting porcine endometrium. Cell Tissue Res. 270, 1–6 (1992). https://doi.org/10.1007/BF00381873

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  • DOI: https://doi.org/10.1007/BF00381873

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