Skip to main content
Log in

Veränderungen des Plasmalemmas während der Zellwandregeneration an isolierten Protoplasten aus dem Sproßkallus von Skimmia japonica Thunb

Structural changes of the plasmalemma during cell wall regeneration of isolated protoplasts from stem callus of Skimmia japonica thunb

  • Published:
Planta Aims and scope Submit manuscript

Summary

The structure of the plasmalemma of isolated protoplasts from stem callus cultures of Skimmia japonica was investigated during cell wall regeneration by means of freeze-etching and deep-etching. Three of the four theoretically possible views of the membrane—the two corresponding inner fracture faces and the outer surface—were made visible. In all cases structural alterations were found in the arrangement of the particles, which are probably responsible for the synthesis and deposit of the cellulose microfibrils.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literatur

  • Branton, D., Southworth, D.: Fracture faces of frozen Chlorella and Saccharomyces cells. Exp. Cell Res. 47, 648–653 (1967)

    Google Scholar 

  • Burgess, J., Fleming, E. N.: Ultrastructural observations of cell wall regeneration around isolated tobacco protoplasts. J. Cell Sci. 14, 439–449 (1974)

    Google Scholar 

  • Grout, B. W. W.: Cellulose microfibril deposition at the plasmalemma surface of regenerating tobacco mesophyll protoplasts: A deep-etch study. Planta (Berl.) 123, 275–282 (1975)

    Google Scholar 

  • Kopp, F.: Zur Membranstruktur: Lokalisation von Membranlipiden im Hefeplasmalemma. Cytobiologie 6, 287–317 (1972)

    Google Scholar 

  • Nagata, T., Takebe, J.: Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta (Berl.) 92, 301–308 (1970)

    Google Scholar 

  • Prat, R., Roland, J.: Étude ultrastructurale des premièrs stades de neoformation d'une enveloppe par les protoplastes végétaux sépares méchaniquement de leur parvi. C. R. Acad. Sci. (Paris) 273, 165–168 (1971)

    Google Scholar 

  • Robinson, D. G., Preston, R. D.: Plasmalemma structure in relation to microfibril biosynthesis in Oocystis. Planta (Berl.) 104, 234–246 (1972)

    Google Scholar 

  • Roland, J., Prat, R.: Les protoplastes et quelques problémes concernant le rôle et l'élaboration des parvis. Colloques int. C.N.R.S. 212, 243–271 (1973)

    Google Scholar 

  • Takebe, J., Nagata, T.: Culture of isolated tobacco mesophyll protoplasts. Colloques int. C.N.R.S. 212, 185–187 (1973)

    Google Scholar 

  • Willison, J. H. M., Cocking, E. C.: The production of microfibrils at the surface of isolated tomato-fruit protoplasts. Protoplasma (Wien) 75, 397–403 (1972)

    Google Scholar 

  • Willison, J. H. M., Cocking, E. C.: Microfibril synthesis at the surfaces of isolated tobacco mesophyll protoplasts, a freeze-etch study. Protoplasma (Wien) 84, 147–159 (1975)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Robenek, H., Peveling, E. Veränderungen des Plasmalemmas während der Zellwandregeneration an isolierten Protoplasten aus dem Sproßkallus von Skimmia japonica Thunb. Planta 127, 281–284 (1975). https://doi.org/10.1007/BF00380725

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00380725

Navigation