Summary
O2-evolution in N2 by young cells of synchronous Ankistrodesmus braunii was measured manometrically in the presence and in the absence of nitrate or nitrite. Nitrate-starved cells produce O2 as a function of nitrate concentration with an optimum at 10 mM nitrate. The optimum rates are strongly dependent upon the pH of the medium culminating at pH 8.0.
Nitrite excretion is initially slow and has its optimum at the same nitrate concentration as O2-evolution. It is slower under anaerobic conditions than in the presence of CO2 and slower at pH 5.6 than at pH 8.0. At pH 8.0 in N2 an accumulation of nitrite starts 40 to 100 min after the addition of nitrate to the algae.
O2-evolution is faster with nitrite than with nitrate. No optimum curve is observed at pH 8.0 with various concentrations of nitrite in the medium; however, at pH 5.6 a distinct peak is found at 3 mM nitrite. This peak is found for a fast short-time reaction as well as for the following low rates of O2-evolution.
The uncoupler carbonylcyanide-m-chlorophenylhydrazone (CCCP) equally inhibits nitrate- and nitrite-dependent O2-evolution and the incorporation of 32P into cellular phosphate compounds. There is no indication of uncoupling in vivo of nitrite reduction which is completely independent of ATP in vitro. The inhibition of the nitrate-dependent O2-evolution by high concentrations of nitrate cannot be explained by an accumulation of products such as ammonia or nitrite.
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Abbreviations
- CCCP:
-
Carbonylcyanid-m-chlorphenylhydrazon
- HEPES:
-
N-2-Hydroxyäthylpiperazin-N-2-äthansulfonsäure
- MES:
-
2-(N-Morpholin)äthansulfonsäure
- Tris:
-
(hydroxymethyl) aminomethan
Literatur
Ahmed, J., Morris, J.: Inhibition of nitrate and nitrite reduction by 2,4-dinitrophenol in Ankistrodesmus. Arch. Mikrobiol. 57, 219–224 (1967)
Beevers, H., Hageman, R. H.: Nitrate reduction in higher plants. Ann. Rev. Plant Physiol. 20, 495–522 (1969)
Davis, E. A.: Nitrate reduction by Chlorella, Plant Physiol. 28, 539–544 (1953)
Domanski-Kaden, J., Simonis, W.: Veränderungen der Phosphatfraktionen, besonders des Polyphosphats, bei synchronisierten Ankistrodesmus-Kulturen. Arch. Mikrobiol. 87, 11–28 (1972)
Grant, B. R., Canvin, D. T.: The effect of nitrate and nitrite on oxygen evolution and carbon-dioxide assimilation and the reduction of nitrate and nitrite by intact chloroplasts. Planta (Berl.) 95, 227–246 (1970)
Grant, B. R., Turner, T. M.: Light-stimulated nitrate and nitrite assimilation in several species of algae. Comp. Biochem. Physiol. 29, 995–1004 (1969)
Herrera, J., Paneque, A., Maldonado, J. M., Barea, J. L., Losada, M.: Regulation by ammonia of nitrate reductase synthesis and activity. Biochem. biophys. Res. Commun. 48, 996–1003 (1972)
Hofmann, A.: Über die energetische Kopplung der Nitratassimilation von Grünalgen an Atmung und Photosynthese. Planta (Berl.) 102, 72–84 (1972)
Jetschmann, K., Solomonson, L. P., Vennesland, B.: Activation of nitrate reductase by oxidation. Biochim. biophys. Acta (Amst.) 275, 276–278 (1972)
Kessler, E.: Untersuchungen zum Problem der photochemischen Nitratreduktion in Grünalgen. Planta (Berl.) 49, 503–523 (1957)
Kessler, E.: Nitrate assimilation by plants. Ann. Rev. Plant Physiol. 15, 57–72 (1964)
Kessler, E.: Stoffwechsel anorganischer N-Verbindungen. Fortschr. Bot. 33, 95–103 (1971)
Kessler, E., Hofmann, A., Zumft, W.: Inhibition of nitrite assimilation by uncouplers of phosphorylation. Arch. Mikrobiol. 72, 23–26 (1970)
Kessler, E., Zumft, W. G.: Effect of nitrite and nitrate on chlorophyll fluorescence in green algae. Planta (Berl.) 111, 41–46 (1973)
Kimimura, M., Katoh, S., Ikegami, I., Takamiya, A.: Inhibitory site of carbonylcyanide m-chlorophenylhydrazone in the electron transfer system of the chloroplasts. Biochim. biophys. Acta (Amst.) 234, 92–102 (1971)
Losada, M., Herrera, J., Maldonado, J. M., Paneque, A.: Mechanism of nitrate reductase reversible inactivation by ammonia in Chlamydomonas. Plant Science Letters 1, 31–37 (1973)
Maldonado, J. M., Herrera, J., Paneque, A., Losada, M.: Reversible inactivation by NADH and ADP of Chlorella fusca nitrate reductase. Biochem. biophys. Res. Commun. 51, 27–33 (1973)
Morris, J., Ahmed, J.: The effect of light on nitrate and nitrite assimilation by Chlorella and Ankistrodesmus. Physiol. Plantarum (Cph.) 22, 1166–1174 (1969)
Pirson, A., Ruppel, H. G.: Über die Induktion einer Teilungshemmung in synchronen Kulturen von Chlorella. Arch. Mikrobiol. 42, 299–309 (1962)
Syrett, P. J.: The assimilation of ammonia and nitrate by nitrogen-starved cells of Chlorella vulgaris. II. Physiol. Plantarum (Cph.) 9, 19–27 (1956)
Ullrich, W. R.: Zur Wirkung von Sauerstoff auf die 32P-Markierung von Polyphosphaten und organischen Phosphaten bei Ankistrodesmus braunii im Licht. Planta (Berl.) 90, 272–285 (1970)
Ullrich, W. R.: Nitratabhängige nichtcyclische Photophosphorylierung bei Ankistrodesmus braunii in Abwesenheit von CO2 und O2. Planta (Berl.) 100, 18–30 (1971)
Ullrich, W. R.: Comparative estimation of cyclic and noncyclic photophosphorylation in N2 by Ankistrodesmus braunii in vivo. Proc. 2nd Internat. Congress on Photosynthesis Res. Vol. 2, 1329–1336 (1972)
Ullrich-Eberius, C. L.: Beziehungen der Aufnahme von Nitrat, Nitrit und Phosphat zur photosynthetischen Reduktion von Nitrat und Nitrit und zum ATP-Spiegel bei Ankistrodesmus braumii. Planta (Berl.) 115, 25–36 (1973)
Warburg, O., Negelein, E.: Über die Reduktion der Salpetersäure in grünen Zellen. Biochem. Z. 110, 66–115 (1920)
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Herrn Prof. Dr. O. Stocker zum 85. Geburtstag gewidmet. — Aus dem Botanischen Institut I der Universität Würzburg.
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Ullrich, W.R. Die nitrat- und nitritabhängige photosynthetische O2-Entwicklung in N2 bei Ankistrodesmus braunii . Planta 116, 143–152 (1974). https://doi.org/10.1007/BF00380649
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DOI: https://doi.org/10.1007/BF00380649