Abstract
In 6 h germinated wheat (Triticum aestivum L. cv. Cama) embryos, more than half of the messenger RNAs are actively involved in translation. Neither preformed nor newly synthesized poly A+-RNA is translated preferentially. Germination in the presence of cordycepin showed that the half-life of the templates is about 2 h and that the newly synthesized messengers are essential to support protein synthesis in the embryo from the first hours of germination. Most of the messenger RNAs in 6 h germinated embryos are newly synthesized. The polypeptides coded for by either the endogenous messenger ribonucleoproteins or purified poly A+-RNA from both dry and germinated embryos are qualitatively identical; minor quantitative differences can however be observed.
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Abbreviations
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
- EDTA:
-
ethylenediaminetetraacetic acid
- SDS:
-
sodium dodecyl sulfate
- TCA:
-
trichloroacetic acid
- mRNP:
-
messenger ribonucleoprotein
- poly A+-RNA:
-
polyadenylic acid containing RNA
- PB:
-
polysome buffer
- GM:
-
germination medium
References
Ajtkhozhin, M., Akhanov, A., Doshchanov, Kh.: Informosomes of germinating wheat embryos. FEBS Lett. 31, 104–106 (1973)
Aviv, H., Leder, P.: Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Natl. Acad. Sci. USA 69, 1408–1412 (1972)
Brawerman, G.: The isolation of messenger RNA from mammalian cells. Meth. Enzymol. 30, 605–612 (1974)
Brooker, J., Tomaszewski, M., Marcus, A.: Preformed messenger RNAs and early wheat embryo germination. Plant Physiol. 61, 145–149 (1978)
Carlier, A., Peumans, W.: The rye embryo system as an alternative to the wheat system for protein synthesis in vitro. Biochim. Biophys. Acta 447, 436–444 (1976)
Chen, D., Sarid, S., Katchalski, E.: Studies on the nature of messenger RNA in germinating wheat embryos. Biochemistry 60, 902–909 (1968)
Delseny, M., Aspart, L., Guitton, Y.: Disappearance of stored polyadenylic acid mRNA during early germination of radish (Raphanus sativus L.) embryo axes. Planta 135, 125–128 (1977)
Delseny, M., Peralta, M., Guitton, Y.: Effects of cordycepin on RNA metabolism in germinating seedlings. Biochem. Biophys. Res. Commun. 64, 1278–1285 (1975)
Dobrzanska, M., Tomaszewski, Z., Grzelczak, Z., Rejman, E., Buchowicz, J.: Cascade activation of genome transcription in wheat. Nature 244, 507–509 (1973)
Doshchanov, Kh., Ajtkhozhin, M., Darkanbaev, T.: Synthesis of cytoplasmic RNAs at the early stages of germination of wheat embryos. Physiol. Rast. 22, 368–375 (1975); Translated in: Sov. Plant Physiol. 22, 305–310 (1975)
Hecker, M., Wiedmann, M., Köhler, H., Serfling, E.: Untersuchungen über den Start der RNA- und Proteinbiosynthesen quellender Agrostemma githago-Embryonen. Biochem. Physiol. Pflanzen 169, 427–436 (1976)
Laskey, R., Mills, A.: Quantitative film detection of [3H] and [14C] in polyacrylamide gels by fluorography. Eur. J. Biochem. 56, 335–341 (1975)
Payne, P.: The long-lived messenger RNA of flowering-plant seeds. Biol. Rev. 51, 329–363 (1976)
Peumans, W., Carlier, A.: Messenger ribonucleoprotein particles in dry wheat and rye embryos. Planta 136, 195–201 (1977)
Peumans, W., Carlier, A., Caers, L.: Sedimentation properties of preformed messenger particles in dry rye embryo extracts. Planta 140, 171–176 (1978)
Spiegel, S., Marcus, A.: Polyribosome formation in early wheat embryo germination independent of either transcription or polyadenylation. Nature 256, 228–230 (1975)
Spiegel, S., Obendorf, R., Marcus, A.: Transcription of ribosomal and messenger RNAs in early wheat embryo germination. Plant Physiol. 56, 502–507 (1975)
Stoddart, J., Thomas, H., Robertson, A.: Protein synthesis patterns in barley embryos during germination. Planta 112, 309–321 (1973)
Takahashi, N., Takaiwa, F., Fukuei, K., Sakamaki, T., Tanifuji, S.: Appearance of newly formed mRNA and rRNA as ribonucleoprotein particles in the cytoplasmic subribosomal fraction of pea embryos. Plant Cell Physiol. 18, 235–246 (1977)
Taneja, S., Sachar, R.: Conserved message of o-diphenolase in wheat embryos (Tricticum aestivum). Experimentia 31, 1128–1130 (1975)
Taneja, S., Sachar, R.: Enzyme synthesis by conserved messengers in germinating wheat embryos. Phytochemistry 15, 1589–1594 (1976)
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Caers, L.I., Peumans, W.J. & Carlier, A.R. Preformed and newly synthesized messenger RNA in germinating wheat embryos. Planta 144, 491–496 (1979). https://doi.org/10.1007/BF00380128
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DOI: https://doi.org/10.1007/BF00380128