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Correlation between an HLA-DQα length polymorphism of messenger RNA and serologically defined specificities (DQwl, DRw53, DR3 + 5)

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Abstract

mRNAs for the two chains of the HLA-DQ molecule were analyzed, in particular the DQα mRNA whose polymorphism had previously been suggested (Schenning et al. 1984). Northern blot transfers of the mRNA of 12 LCLs and of B lymphocytes from a healthy donor were carried out. We report that a length polymorphism of DQα mRNA exists, and we show that it can be correlated with serologically defined specificities (DQwl, DRw53, DR3 + 5). This correlation could be explained by a linkage disequilibrium, as these specificities are considered to be different from those carried by the DQ molecule (except for the DQw1 specificity).

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Abbreviations

DNA:

desoxyribonucleic acid

HLA:

human leukocyte antigen

LCL:

lymphoblastoid cell line

mRLP:

messenger RNA length polymorphism

mRNA:

messenger ribonucleic acid

RFLP:

restriction fragment length polymorphism

RNA:

ribonucleic acid

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Authors and Affiliations

  1. Hôpital Saint-Louis (INSERM U93 and Laboratoire d'Immunologie et d'Histocompatibilité), 2, place du Dr. Fournier, 75475, Paris Cédex 10, France

    Pascale Loiseau, Pierre Lehn, Virginia Lepage, Jacques Colombani, Daniel Cohen & Laurent Degos

  2. Laboratoire d'Oncologie moléculaire UA 612 CNRS, Institut Gustave Roussy, rue Camille Desmoulins, 94800, Villejuif, France

    Pierre Lehn & François Dautry

  3. College de France-Centre d'Etudes du Polymorphisme Humain (CEPH), 3, rue d'Ulm, 75005, Paris, France

    Jean Dausset

Authors
  1. Pascale Loiseau
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  2. Pierre Lehn
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  3. François Dautry
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  4. Virginia Lepage
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  5. Jacques Colombani
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  6. Daniel Cohen
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  7. Jean Dausset
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  8. Laurent Degos
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Loiseau, P., Lehn, P., Dautry, F. et al. Correlation between an HLA-DQα length polymorphism of messenger RNA and serologically defined specificities (DQwl, DRw53, DR3 + 5). Immunogenetics 23, 111–114 (1986). https://doi.org/10.1007/BF00377970

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  • DOI: https://doi.org/10.1007/BF00377970

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