Abstract
An H-2-specific monoclonal antibody (mAb Q-1) was obtained from B10.Q (H-2 q) mice injected with syngeneic Sendai virus-coated cells. The IgM monoclonal antibody recognizes the public determinant H-2.25 shared by H-2 k (K k) and H-2 r haplotypes and cross-reacts with H-2d, H-2s, H-2p, and H-2q cells, the latter being the haplotype of the challenged B-cell donor. The binding of mAb Q-1 to H-2d, H-2s, H-2q, and H-2p cells was lower than to H-2k and H-2r and of decreasing affinity but could be clearly distinguished from the negative reactions with H-2b and H-2f cells. MAb Q-1 distinguishes between Sendai virus-coated and uncoated lymphocytes only cells with low-affinity binding. On virus-coated or infected (H-2p, H-2q, H-2d, H-2s) cells lysis was stronger than on normal lymphocytes. We interpret the enhanced lysis of Sendai virus-positive cells by mAb Q-1 to be due to recognition of a modified exposure of public H-2 determinants induced by Sendai virus.
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Černý-Provazník, R. and Iványi, P.: Frequency of naturally occurring H-2-specific antibodies in mouse sera by “superreactive” rabbit complement. Tissue Antigens 26: 259–261, 1985
Černý-Provazník, R., Radl, J., Leupers, T., van Mourik, P., Brondijk, R., de Greeve, P., and Iványi, P.: Anti-MHC immunity detected prior to intentional alloimmunization. I. Naturally occurring H-2-specific antibodies in C57BL/KaLwRij (H-2b) mice. Nat. Immun. Cell Growth Regul. 4: 138–159, 1985a
Černy-Provazník, R., van Mourik, P., Radl, J., Leupers, T., Limpens, J. C., and Iványi, P.: Anti-MHC immunity detected prior tointentional alloimmunization. II. Monoclonal H-2-specific antibodies obtained from a normal C57BL/KaLwRij (H-2b) mouse. Nat. Immun. Cell Growth Regul. 4: 160–168, 1985b
Černý-Provazník, R., van Mourik, P., Limpens, J., Leupers, T., and Iványi, P.: Anti-MHC immunity detected prior to intentional alloimmunization. HI. Natural autoreactive H-2-specific antibodies. Immunogenetics 21: 491–504, 1985c
Černý-Provazník, R., Kloosterman, T., Verkerk, D., Vroom, T. M., and Iványi, P.: Natural autoreactive H-2-specific serum antibodies in a group of BALB/cBy (H-2d) mice. Tissue Antigens 27: 106–111, 1986a
Černý-Provazník, R., Kloosterman, T., Verkerk, D., van Mourik, P., Reboul, M., Frangoulis, B., Pla M., and Iványi, P.: Anti-MHC immunity detected prior to intentional alloimmunization. IV. Natural monoclonal H-2-specific antibodies. J. Immunogenet., in press, 1986b
Dick, H. N., Bodmer, W. F., Bodmer, J. G., Steel, C. M., Crichton, W. B., and Evans, J.: HLA typing of lymphoblastoid cell lines. In F. Kissmeyer-Nielsen (ed.): Histocompatibility Testing 1975, pp. 671–675, Munksgaard, Copenhagen, 1975
Flaherty, L. and Lynes, M. A.: Use of monoclonal antibodies in the study of the fine specificity of antigens coded for by the major histocompatibility complex in mice. In G. Di Sabato,, J. J. Langore, and H. V. Vunokis (eds.): Methods in Enzymology, Volume 108, pp. 481–494, Academic Press, New York, 1984
Günther, E., Elsasser-Beile, U., and Hedrich, H. J.: Occurrence of antibodies against RT1.A antigens in normal rat sera. Transplant. Proc. 15: 1560–1561, 1983
Iványi, P., van den Berg-Loonen, E., and de Greeve, P.: Individual mice of one inbred strain produce anti-H-2 and anti-HLA antibodies of different specificities. Tissue Antigens 12: 32–38, 1978
Iványi, P., Melief, C. J. M., van Mourik, P., Vlug, A., and de Greeve, P.: Lymphocytotoxic antibodies produced by H-2 allo-immunisation distinguish between MuLV positive and -negative substrains of the same H-2 haplotype. Nature 282: 843–845, 1979
Iványi, P., van Mourik, P., Breuning, M., Kruisbeek, A. M., and Kröse, C. J. M.: Natural H-2-specific antibodies in sera of aged mice. Immunogenetics 15: 95–102, 1982
Kast, W. M., de Waal, L. P., and Melief, C. J. M.: Thymus dictates major histocompatibility complex (MHC) specificity and immune response gene phenotype of class II MHC-restricted T cells but not of class I MHC-restricted T cells. J. Exp. Med. 160: 1752–1766, 1984
Kievits, F., Opolski, A., Limpens, J., Rocca, A., Leupers, T., Kloosterman, T., Boerenkamp, W. J., Pla, M., and Iványi, P.: Induction of H-2-specific antibodies by injections of syngeneic Sendai virus-coated cells. Eur. J. Immunol., in press, 1986
Kloting, I. and Stark, O.: Occurrence of antibodies against gene products of the major histocompatibility complex in normal sera of diabetesprone BB rats. Folia Biol. (Praha) 31: 34–45, 1985
Levy, R. B., Dower, S. K., Shearer, G. M., and Segal, D. M.: Trinitrophenyl modification of H-2k and H-2b spleen cells results in enhanced serological detection of Kk-like determinants. J. Exp. Med. 159: 1464–1472, 1984
Longenecker, B. M. and Mosmann, T. R.: “Natural” antibodies to chicken MHC antigens are present in mice, rats, humans, alligators and allogeneic chickens. Immunogenetics 11: 293–302, 1980
Mann, D. L., Popovic, M., Murray, S. P., Reitz, M. S., Strong, D. M., Haynes, B. F., Gallo, R. C., and Blattner, W. A.: Cell lines producing human Tcell lymphoma virus show altered HLA expression. Nature 305: 58–59, 1983
McMillan, N., Levis, K. D., and Rovner, D. M.: Molecular characterization of novel H-2 class I molecules expressed by a C3H UV induced fibrosarcoma. Proc. Natl. Acad. Sci. U.S.A. 82: 5485–5489, 1985
Mierau, R. and Cramer, M.: Serological and immunochemical analysis of H-2 class I molecules encoded by the D b region. Immunogenetics 20: 341–345, 1984
Muller, C. P., Stephany, D. A., Shinitzky, M., and Wunderlich, J. R.: Changes in cell surface expression of MHC and Thy-1.2 determinants following treatment with lipid modulating agents. J. Immunol. 131: 1356–1363, 1983
Muller, C. P., Stephany, D. A., and Wunderlicht, J. R.: Changes in mouse MHC expression during the cell cycle of ConA-treated spleen cells. J. Immunol. 136: 17–22, 1986
Ozato, K., Mayer, N., and Sachs, D. H.: Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533–540, 1980
Philipps, C., McMillan, M., Flood, P. M., Murphy, D. B., Forman, J., Lancki, D., Womack, J. E., Goodenow, R. S., and Schreiber, H.: Identification of a unique tumor-specific antigen as a novel class I major histocompatibility molecule. Proc. Natl. Acad. Sci. U.S.A. 82: 5140–5144, 1985
Sherman, L. A., Vitiello, A., and Klinman, N. R.: T -cell and B-cell responses to viral antigens at the clonal level. Annu. Rev. Immunol. 1: 63–86, 1983
Stinchcombe, V., Jones, T., and Bradley, R. A.: HLA typing of EpsteinBarrvirus transformed lymphoblastoid cell lines (CLC). TissueAntigens 26: 161–167, 1985
Tongio, M. M., Falkenrodt, A., Mitsnishi, Y., Urlacher, A., Bergera, J. P., North, M. L., and Mayer, S.: Natural HLA antibodies. Tissue Antigens 26: 271–285, 1985
van Leeuwen, A., Goulmy, E., and van Rood, J. J.: Major histocompatibility complex-restricted antibody reactivity mainly, but not exclusively, directed against cells from male donors. J. Exp. Med. 150: 1075–1083, 1979
van Mourik, P. and Zeijlemaker, W. P.: Improved hybridoma technology: Spleen cell preparation and soluble growth factor. In S. P. Colowich and N. O. Kaplan (eds.): Methods in Enzymology, Volume 121, pp. 174–200, Academic Press, Orlando, 1986
van Mourik, P., Rivero, R. A., van der Kwast, T. H., Lansdorp, P. M., and Zeijlemaker, W. P.: Density separation of spleen cells increases fusion frequency and yield of Ig-producing hybridomas. J. Immunol. Methods 68: 45–55, 1984
Wylie, D. E., Sherman, L. A., and Klinman, N. R.: Participation of the major histocompatibility complex in antibody recognition of viral antigens expressed on infected cells. J. Exp. Med. 155: 403–414, 1982
Yang, H. and Welsh, R. M.: Induction of alloreactive cytotoxic T cells by acute virus infection of mice. J. Immunol. 136: 1186–1193, 1986
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On leave from The Institute of Immunology and Experimental Therapy, Wroclaw, Poland
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Opolski, A., Kievits, F. & Iványi, P. Polymorphic and autoreactive H-2-specific monoclonal antibody isolated after injections of syngeneic sendai virus-coated lymphocytes. Immunogenetics 24, 402–408 (1986). https://doi.org/10.1007/BF00377959
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DOI: https://doi.org/10.1007/BF00377959